Highly sensitive method for detection of viral hiv dna remaining after antiretroviral therapy of aids patients

ABSTRACT

Methods for detecting polynucleotides, especially the DNA replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodefiency virus, by detecting electromagnetic signals (“EMS”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“PCR”).

CROSS-REFERENCE TO RELATED APPLICATION(S)

This application claims priority under 35 U.S.C. §119(e) to U.S. Provisional 61/186,610, filed Jun. 12, 2009, which is incorporated by reference in its entirety.

BACKGROUND OF THE INVENTION

1. Field of the Invention

Methods for detecting polynucleotides, especially the DNA replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodeficiency virus, by detecting electromagnetic signals (“EMS”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“PCR”). Electromagnetic signals of low frequency have been shown to be produced in aqueous dilutions by Human Immunodeficiency Virus DNA. In vivo, HIV DNA signals are detected only in patients previously treated by antiretroviral therapy and having no detectable viral RNA copies in their blood. It is suggested that the treatment of AIDS patients pushes the virus towards a new mode of replication implying only DNA, thus forming a reservoir insensitive to retroviral inhibitors. Implications for new approaches aimed at eradicating HIV infection are disclosed.

2. Description of the Related Art

Antiretroviral therapy (ART) is now the standard treatment of HIV infected patients. Generally composed of three or four inhibitors of the viral reverse transcriptase and protease, it results in a quasi complete disappearance of HIV viremia, or measured by the strong reduction of viral RNA copies (viral load) in the patient's serum. The limit of detection of RNA copies by commercial kits (200 virus/ml or 40 virus/ml) is usually attained within 3 to 6 months when the virus is fully sensitive to the viral inhibitors. However, as soon as the treatment is interrupted, virus multiplication resumes within weeks, as evidenced by the increase of the virus load and the decrease of the CD4 T-cell numbers.

This indicates that there is a viral reservoir to which the inhibitors have no access or no effect. This reservoir is presumably made of proviral DNA integrated in cells in a dormant state. It is shown that ART treatment of patients induces the release into their blood of HIV DNA sequences detectable by a new biophysical technology. The data suggests that inhibition of infection at the reverse transcription step is pushing the virus towards a low level of replication using only DNA templates. This would explain why the classical inhibitors used in ART cannot achieve eradication of the viral infection.

Detection of electromagnetic waves of low frequency by high dilutions in water of the DNA of pathogenic bacteria has been previously reported. This is a resonance phenomenon likely to be produced by polymerized water molecules organized by some DNA sequences. It has been contemplated that the genetic material of viruses, particularly that of HIV, could also induce the same transformation of water.

Pathogenic microorganisms in this day of age are not only submitted to high selective pressure by the immune defenses of their hosts but also have to survive under highly active antiviral treatments. Not surprisingly, they have evolved in finding many ways to escape these hostile conditions, such as mutations of resistance, hypervariability of surface antigens, protective biofilms, latency inside cells and tissues. It has been observed that some filtration procedures aimed at sterilizing biological fluids can yield under some defined conditions the infectious microorganism which was present before the filtration step. A 20 nM filtration did not retain a minor infective fraction of HIV, the causal agent of AIDS, whose viral particles have a diameter averaging 100-120 nM. In the course of investigating the nature of such filtering infectious forms, another property of the filtrates was found that may or may not be related to the former: their capacity to produce some electromagnetic waves of low frequency in a reproducible manner after appropriate dilutions in water.

The emission of such waves is likely to represent a resonance phenomenon depending on excitation by the ambient electromagnetic noise. It is associated with the presence in the aqueous dilutions of polymeric nanostructures of defined size. The supernatant of uninfected eukaryotic cells used as controls did not exhibit this property. Disclosed is a first characterization of the electromagnetic signals (EMS) and of their underlying nanostructures produced by some purified viruses.

BRIEF SUMMARY OF THE INVENTION

The disclosed invention includes:

A method of detecting electromagnetic signals (EMS) emitted by genes of viruses, in particular genes from pathogenic viruses. Also, a method of detecting electromagnetic signals emitted by DNA or specific nucleotide sequences. The methods include steps of filtering, diluting, and vortexing of samples of body fluids, tissues or cells; or samples of DNA extracted from body fluids, tissues or cells. The samples are serially diluted with the samples being vigorously vortexed between each dilution step.

A method of improving the sensitivity of PCR by 10 to 100 times by processing samples with serial dilutions (1/10 at each step) and vigorous vortexing between each dilution step. Additionally, RNase treatment of the filtered original sample can be combined with the serial dilution process.

A composition of viral genes, specific nucleotide sequences or DNA, in general, that is able to emit EMS when the appropriate dilution of the sample is obtained by serial dilution and vortexing between each dilution step.

A machine to process biological samples to automatically make the initial solution of biological fluid or solution of DNA extracted from a biological sample of body fluid, tissue or cells; filter the original solution followed by serial dilutions of the sample with vortexing of the diluted sample before the next serial dilution; and detecting, measuring and analyzing an emitted EMS to determine if it corresponds to an EMS from a specific pathogenic virus or gene.

A machine to detect a pathogenic infection in a human or animal by a non-invasive method and detecting, measuring and analyzing an emitted EMS from a body part placed on a scanner surface. The detected EMS would be compared to an EMS indicative of the specific pathogenic infection.

Additional embodiments of the disclosed invention are described below.

BRIEF DESCRIPTION OF THE DRAWINGS EMS in “Silent” and “Loud” Samples

FIG. 1 shows a typical background EMS detected in an unfiltered suspension or a negative low dilution, and how this background noise appears after it has been analyzed with Fourier transformation, graphic representation, and harmonics. Panel A shows an EMS with large changes in the amplitude of the signal with small changes in frequency and small ranges between the high and low values. Panel B shows that the spikes are very small on the right side of the graph. Panel C shows only small peaks near the origin and spaced along the graph.

FIG. 2 shows a typical EMS recording from the plasma DNA of a patient positive for HIV and who has received antiretroviral therapy. Panel A shows a very different signal pattern. The amplitude of the signal remains relatively constant with a higher frequency of spikes and a large range between the high and low value of each spike. Panel B shows that the spikes are very large on the right side of the graphs. Panel C now shows large spikes near the origin with large spikes found all along the base of the graph.

Stability of EMS in Sample

FIG. 3 shows the EMS emission from a sample of plasma from a patient positive for HIV and who has received antiretroviral therapy. The sample has been stored at 4° C. The EMS recording on Day 0 has the typical appearance of only having background EMS with the right side of the graph having small spikes. When the same sample has the EMS recorded on Day 14 and Day 34, there is no change in the EMS and it looks just like the recording taken on Day 0.

FIG. 4 shows the EMS emission from a sample of plasma from a patient positive for HIV and who has received antiretroviral therapy. The sample has been stored at 4° C.; however it has been filtered and diluted to 10⁻⁵ in serial steps of 1 part sample solution: 9 parts diluent (decimal dilution) with each step consisting of dilution followed by vigorous and sustained vortexing of the prepared diluted solution. The sample was diluted in steps from the original sample to 10⁻¹, 10⁻², 10⁻³, 10⁻⁴, and finally to 10⁻⁵. The EMS was recorded from the 10⁻⁵ dilution. The EMS recorded on Day 0 has very large peaks on the right side of the graph as compared to the peaks on the left side of the graph. The EMS recorded on Day 14 still has large peaks with the range of the peaks expanded a little more compared to the recording on Day 0. The EMS recording on Day 34 is very similar to Day 14 and still shows the typical pattern seen in a sample emitting an EMS from the HIV virus. The stability of the EMS generating entity appears to persist for many days and weeks in some samples.

EMS Signal Versus Dilution of the Sample

FIG. 5 shows the EMS recording from the serial dilutions of a sample of plasma DNA from a patient positive for HIV and who has received antiretroviral therapy. EMS recordings were taken from each serial dilution from beginning with the original not filtered (NF) sample through the first dilution of 10⁻¹ to the last dilution of 10⁻¹2. Also, it must be noted that each serial dilution was vigorously vortexed before making the next dilution in series. The EMS signal in the non-filtered sample has the appearance of background noise with none of the typical changes in the signal pattern seen in an EMS emitting sample. Sample D5 (10⁻⁵ dilution) begins to show the typical pattern of an EMS emitting sample and this pattern continues in the samples until D8 (10⁻⁸). Sample D9 (10⁻⁹) shows that the EMS pattern has reverted back to that seen in the non-filtered sample that is typical of background noise. This pattern continues in the samples up to D12 (10⁻¹²). These graphs show that low dilutions and very high dilutions do not emit EMS. It is only the dilutions in the range of 10⁻⁵ to 10⁻⁸ that have the detectable EMS.

FIG. 6 shows the Fourier transformation analysis of the same samples as in FIG. 5. These graphs more easily show the EMS from the samples. Samples NF to D4 do not show any large peaks on the right side of the graph. However, beginning with sample D5 and ending with sample D8, the graphs show large peaks on the right side of the graphs indicating the emission of EMS from the EMS generating entity associated with HIV infection. Finally samples D9 to D12 reverted back to the pattern seen with background noise.

FIG. 7 shows the Fourier transformation analysis presented with spikes rather than waves as in FIG. 6. These graphs allow an easier analysis of the EMS from the samples. Samples NF to D4 and D9 to D12 do not show any spikes in the graph, which indicates the recording of just background noise. However, samples D5 to D8 show many spikes near the origin and multiple spikes along the base of the graph. This pattern shows the EMS emission indicating the presence of nanostructures induced by HIV DNA.

EMS Recording Apparatus

FIG. 8 shows a schematic representation of the equipment used to record EMS from samples. A coil, bobbin of copper wire, surrounded the sample vial to detect the EMS. The wires from this coil were connected to a Sound Blaster Card, which in turn was connected to a laptop computer. A laptop computer is preferred since it can be run with battery power, which eliminates some background EMS from AC current. Each emission was recorded twice for 6 seconds, amplified 500 times and processed with different softwares for visualization of the signals on the computer's screen. The main harmonics of the complex signals were analyzed by utilizing several softwares of Fourier transformation.

Increased Sensitivity of PCR

FIG. 9 is an electrophoretic gel showing the detection of DNA in each of the serial dilutions of a blood sample obtained from a patient positive for HIV and receiving ART. The sample was processed with two different methods: 1) the typical method of mixing the diluted sample between each serial dilution and 2) the improved method of vigorously vortexing the diluted sample between each serial dilution. The diluted samples were run on the gel to detect the DNA present in each dilution. The samples run on the gel were from NF (original undiluted sample) to D I O (10⁻¹⁰). The NF band was the only band visible in the samples that were just mixed between each step of serial dilutions. However, bands were visible for the NF, D2 (10⁻²) and D3 (10⁻³) samples when the samples were vigorously vortexed between each serial dilution. This improved method showed an increase of sensitivity by 100 times over the typical methods used with PCR.

FIG. 10 (Table A): FIG. 10 shows the representative results of an experiment testing for EMS in patients with varying levels of HIV infection and antiretroviral therapy. The presence of EMS was tested in various samples from these patients: unfrozen blood plasma and samples with DNA extracted from a frozen blood sample—Plasma DNA, WBC (white blood cells) DNA and RBC (red blood cells) DNA._The first group of patients (B I-B4) was asymptomatic for HIV and had not received antiretroviral therapy. All samples from these patients, whether the sample was fresh plasma or DNA extracted from frozen blood, did not emit EMS at any dilution. These patients had HIV virus present in their samples, but their presence did not cause the emission of EMS. The second group of patients (C I-C4) was asymptomatic for HIV and had received antiretroviral therapy. These patients' plasma and RBC DNA samples emitted EMS; however the WBC samples did not emit EMS and were silent. Also, it did not depend if the sample was from fresh plasma or DNA extracted from a frozen sample. Additionally, the dilution range for emitting EMS was very similar from sample source (Plasma, Plasma DNA, WBC DNA, and RBC DNA) and between patients (C1-C4). The dilution emitting EMS ranged from D3 (10⁻³) to D9 (10⁻⁹). It is important to note that the WBC DNA did not emit EMS, indicating that the EMS generating entity (probably DNA) was not present in these cells, although some of the WBC [CD4 lymphocytes and monocytes] are the target for the HIV virus. Additionally, the RBC DNA sample was positive for emitting EMS, even though RBC lack a nucleus. It is theorized that the EMS generating entity may be adsorbed to the exterior cell membrane of the RBC or associated with a cell that co-migrates with RBC during fractionation. Also, the EMS generating entity may have the same density as RBC so it found in the RBC fraction. The third group of patients (D1-D4) was symptomatic for HIV and had not received antiretroviral therapy, that is, showing full-blown AIDS. It is difficult to find patients in the Developed World in this condition due to the wide-spread use of antiretroviral therapy. However, it is very common to find patients with untreated AIDS in the Undeveloped World due to high cost of the antiretroviral therapy drugs and lack of money by the patients or their countries. During a trip to Central Africa, samples were obtained from patients with Full-Blown AIDS. Blood samples from these symptomatic untreated patients were found not to emit EMS. None of the samples, whether fresh or from frozen samples, were found to emit EMS. This finding suggests that that the EMS generating entity is only produced when the virus replication has been inhibited by antiretroviral therapy. If the production of this EMS generating entity was just a step in the progression of the HIV infection, it would be expected in patients with a long duration of infection, that is in patients who are asymptomatic for AIDS and had not received antiretroviral therapy and patients who are symptomatic for HIV and had not received antiretroviral therapy, since both groups represent people with long-term HIV infections. The finding that samples from symptomatic untreated patients lacked EMS indicated that the process that produces the EMS generating entity was probably associated with some “self-preservation” mechanism of the HIV virus to hide from the immune system of the infected animal.

FIG. 11 (Table B): FIG. 11 shows representative results of an experiment testing for EMS from DNA bands resulting from PCR and nested-PCR. Five HIV genes (Gag, Pol, Env, LTR, & Net) were amplified, isolated and samples prepared and diluted with serial dilutions. There were differences in the ability to emit EMS found between individual genes and whether the DNA was produced by PCR or nested-PCR. The Gag and Pol genes did not emit EMS from DNA produced by either PCR or nested-PCR. The Env, LTR and Nef genes showed EMS emission when the DNA was obtained using nested-PCR and the sample was diluted from 10⁻⁴ to 10⁻⁸. These results indicated that the EMS may be associated with a specific gene or genes in the HIV virus. However, there may be other genes or nucleic acid sequences that emit EMS.

DETAILED DESCRIPTION OF THE INVENTION

The invention relates to the detection in a blood sample of electromagnetic signals coming from HIV DNA, in patients which have undetectable viral RNA in their blood as measured by commercial existing tests.

This new test will allow elaborating new therapeutics aimed at reducing the amount of this DNA, thus creating the possibility to eradicate the viral infection. In clinical sites, unfrozen plasma from fresh blood samples can be used and directly tested. Otherwise the plasma sample will have to be shipped in a frozen state and DNA can be extracted from this plasma, as well as, from blood cells, including those associated with the erythrocyte fraction, and also from any tissue or body fluid.

The technology of detecting EMS from pathogenic particles was refined and improved to the capture electromagnetic signals of HIV DNA sequences. Unlike the nanostructures induced in water by bacterial DNA, which passed through 100 nM filters but were retained by 20 nM filters, the HIV DNA nanostructures are smaller since they passed through 20 nM filters. The range of dilutions are lower at which the EMS from viruses could be detected, starting from 10⁻³ up to 10⁻⁹ decimal (i.e., 1 part sample:9 parts diluent] dilutions. There is no detectable difference in the profile of the signals at this level of technology, indicating that it is probably due to a resonance phenomenon of water polymers.

The material structures at the origin of the signals are unlikely to come from mature HIV virions, as they differ in density in sucrose gradient from the density of 1.16 of retroviruses. Moreover, in the blood of AIDS patients, they are produced by DNA and not RNA.

An important observation, although paradoxical, is that only HIV-related DNA sequences from patients treated with antiretroviral therapy and having no detectable RNA in their blood can be detected by EMS emission and by PCR. Naïve untreated patients, either with high or low virus load, show no evidence of such DNA. This result was obtained with patients of different geographic locations (North America, Europe, West and Central Africa) presumably infected with different HIV subtypes.

Interestingly, this DNA is not only detected in the plasma fraction, but also found associated with the erythrocyte fraction. As there is no DNA in mature erythrocytes, the viral DNA is probably present in nanostructures bound to the erythrocyte membrane (exosomes) or in nucleated cells that sedimented with the erythrocytes (i.e., granulocytes). In treated patients still having a detectable virus load, the DNA was only found in the plasma fraction.

There are several possible source(s) of this DNA and possible roles by this DNA. PCR analysis of the prototype HIV 1 Lai DNA indicated that short fragments of nested-PCR amplified DNA, in picogram amounts, are the source of EMS, derived in particular from the LTR, Env and Nef genes. Similarly, in the case of patient DNA, the LTR, Nef and Env-derived amplicons were also EMS positive.

There may be in vivo DNA fragments corresponding to other genes which are not picked-up by the primers currently used. Therefore it is possible that the whole genome is represented as DNA fragments in the blood or even as an entire genomic molecule. The most simplistic explanation for the presence of this DNA is that it reflects the breakdown (e.g., apoptosis) of some infected cells containing the proviral DNA in a latent state. This would imply that after antiretroviral treatment, these cells die and constitute a reservoir large enough to be continuously refilled by new living cells. A priori, there is no reason that such cells, unless they express some HIV proteins recognized by cytotoxic T cells, will be destroyed by the immune reactions.

Possibly the DNA detected represents forms of unintegrated HIV DNA. Various circular DNA forms have been described during HIV infection in vitro and in vivo. Sharkey et al. have even described the persistence of episomal forms of HIV DNA in some patients treated by antiretroviral therapy with undetectable viral RNA in their blood. However their study was focalized in peripheral blood mononuclear cells (PBMC). However, our study could not detect HIV DNA in PBMC, indicating it comes from other cell types and tissues. Another possible theory is that the antiretroviral therapy works efficiently to prevent reverse transcription of viral RNA into DNA and therefore blocks any productive infection of susceptible cells. However it will not prevent DNA-DNA replication in a non-integrated state. In other words, the ART treatment pushed the virus towards an alternate way of replication, probably minor and depending on a cellular DNA polymerase, but sufficient to maintain the viral genetic information as unintegrated viral DNA and able to resume the normal viral cycle if ART is interrupted for any reason.

The DNA found in the blood circulation would then be a by-product of this DNA. The cells and tissues in which this DNA replication occurs have not been identified. This theory, if correct, would have some important implications for the eradication of HIV infection. If specific inhibitors can target this episomal replication, without damaging the cellular processes, a complete elimination of the HIV reservoir might be achieved and therefore eradication of HIV infection.

Experiments have indicated that this detection also applies at the scale of the human body: the same EMS has been detected in the plasma and in the DNA extracted from the plasma of patients suffering of Alzheimer, Parkinson disease, multiple Sclerosis and Rheumatoid Arthritis.

The physical nature of the nanostructures which support the EMS resonance remains to be determined. It is known from the very early X-ray diffraction studies of DNA that water molecules are tightly associated with the double helix, and any beginner in molecular biology knows that DNA in water solution forms gels associating a larger number of water molecules. Moreover, a number of physical studies have reported that water molecules can form long polymers of dipoles associated by hydrogen bonds (Ruan et al., 2004; Wernet el al., 2004). However these associations appear to be very short-lived (Cowan et al., 2005).

EXAMPLES Example 1 Measurement of Electromagnetic Signals

The plasma or DNA solution [1-4 ng/ml] is dissolved in Phosphate Buffered Saline (PBS) at the concentration of 10⁻², then filtered on Millipore 0.45 micrometer filter and the filtrate is refiltered on Anotop Whatman filter of 20 nanometer porosity. The filtrate is then diluted in distilled water in 1.5 ml Eppendorf conical plastic tubes in serial 1 part sample: 9 parts diluent [decimal] dilutions ranging from 10⁻² to 10⁻¹⁵ and strongly agitated on a vortex for at least 15 seconds.

Plasma is prepared by centrifugation of heparinized blood of patients presenting with conditions of: 1) Asymptomatic, untreated; 2) Symptomatic, not yet treated, with high virus load; or 3) Symptomatic, treated by antiretroviral therapy with no detectable virus load by commercial kits (<200 RNA copies/ml).

EMS was only detected in the plasma of the third category (30 out of 30), in plasma dilutions ranging from 10⁻⁵ to 10⁻⁸. Results with the two first categories were generally negative, with the exception of one untreated AIDS patient.

The conditions of preparation and storage of the plasma sample was determined for optimizing the capture of EMS. The plasma had to be kept unfrozen, preferentially stored at +4° C. Freezing and storing at −20° C. or −80° C. destroyed their capacity to produce EMS, unless DNA was extracted, the primary source of the signals.

Serum taken from the clotted blood was also negative, whether kept at +4° C. or frozen. Heating the diluted 10⁻² plasma at 65° C. for one hour also inactivated or reduced significantly the EMS.

Example 2 The Decay with Time of EMS Production in Plasma Stored at +4° C.

The capacity to emit EMS in plasma can last for several days, sometimes for several weeks of storage, indicating a relative stability of the nanostructures that emit EMS in the plasma proteinic environment. In vitro studies indicated that filtration of the plasma (usually at the 1/100 dilution in PBS or saline) through 20 nM filters was a prerequisite for detecting the signals in further dilutions of water. In some rare cases, weaker signals can be detected at lower dilutions after filtration through 100 nM porosity filters. Positive signals were usually found in the range of the 10⁻³ to 10⁻⁹ dilutions.

Example 3 Evidence that Positive Signals come from DNA

Experiments were conducted to determine if nucleic acids carrying the genetic information for HIV, either residual viral RNA or proviral DNA, could be the sources of signals in the plasma of infected patients. Three groups of patients: infected and not treated in the asymptomatic stage; infected and not treated in the symptomatic stages; and infected and treated with ART with no detectable viral load.

Plasma was diluted 1/100 in PBS and the nucleic acids were extracted by the phenol-chloroform method. The solution was precipitated with ethanol and the precipitates were solubilized in water. The solution was filtered through a 20 nM filter at a concentration ranging from 1 ng/ml to 4 ng/ml.

EMS emissions were detected only in the group of patients treated by antiretroviral therapy and having an undetectable virus load. The signals were produced in the same range of aqueous dilutions than fresh plasma. Filtration of the original solution (1/100 dilution) and vortex agitation of each of the further aqueous dilutions was necessary in order to capture the EMS emission.

Treatment by RNase (10 μg/ml, 1 hour at 37° C.) of the original solution had no effect. This suggested that DNA, rather than viral RNA, was involved in EMS production. Confirmation was obtained by DNase inactivation. However this only occurred if the sample, which previously had EMS, was frozen and thawed before the DNase treatment. If the sample was not frozen, then the sample would continue to have EMS after DNase treatment. It is believed that nanostructures previously induced by the DNA in the water remain after DNase treatment, if they have not been eliminated by freezing or other treatments that are known to eliminate EMS emitting from samples. However DNA molecules are not affected by freezing and the DNA can re-induce the water nanostructures after the specimen is thawed. The experimental protocol and results are shown in the following table.

TABLE 1 DNA Solution, filtered 450 nM, then 20 nM, 2 ng/ml Control +RNase (10 μg/ml) +DNase (10 U/μg) untreated 10 mM Tris- 10 mM Tris- 10 mM Tris- HCl, pH 7.4 HCl, pH 7.4, HCl, pH 7.4 37° C./2 hours 37° C./2 hours

↓

Freezing −20° C./2 hours

↓

EMS positive positive negative Range of D5-D9 D5-D9 dilutions

Plasma, plasma DNA and erythrocyte DNA were obtained from patients or individuals in different conditions: 1) naive (untreated) positive patients at the asymptomatic stage; 2) naive patients with full blown AIDS and high virus load; 3) AIDS patients treated by antiretroviral therapy (usually 2 nucleosidic reverse transcriptase inhibitors and 1 non nucleosidic reverse transcriptase inhibitor or 1 protease inhibitor) and having undetectable virus load (viral RNA copy number inferior to 40/ml of blood) and 4) uninfected controls. At least 10 patients of each group were tested.

The third group was the only one that showed positive electromagnetic signals, both in fresh plasma or in DNA extracted from frozen plasma. The DNA extracted from the erythrocyte pellet (probably containing some nucleated cells such as granulocytes) was also positive.

If the treated patient still had a high viral load upon treatment, only the plasma DNA was positive. This was also the case of pregnant women treated by viral inhibitors in the last trimester of their pregnancy.

No untreated patient was positive in any of the three fractions: plasma DNA, red cell pellet DNA, and leukocyte layer DNA.

The DNA was identified as representative of HIV DNA by the following:

using an infectious HIV DNA clone, derived from a prototype laboratory strain, and

(HIV 1 Lai) containing all HIV genes, electromagnetic signals were detected from water solution in the same range of dilutions.

Specific Polymerase Chain Reaction (PCR) primers were used for the different gene sequences of HIV DNA (LTR, Pol, Env, Nef) these sequences were amplified from the DNA of patients positive for the electromagnetic signals. After a second round of amplification (nested PCR), these solutions induced the signals at similar dilutions as the whole DNA.

It also was noted that some specific sequences (LTR and to a lower extent Net) were detected by RT-PCR (using reverse transcriptase as first polymerase), which resulted in a higher sensitivity.

Additionally, the obtained DNA bands were of higher intensity and increased sensitivity (10×-100×) when the DNA dilution to be used for amplification was thoroughly vortexed in the same manner as used for preparations used to detect the electro-magnetic signals (EMS).

RNase treatment of the DNA before RT-PCR (10 μg/ml, 2 hours, 37° C.) did not affect the results. This observation indicated that the reverse transcriptase was not using RNA, but a DNA template or another template as yet unidentified.

The detection of HIV DNA only in patients treated with antiretroviral therapy and having undetectable viral RNA in their blood indicated that the antiretroviral therapy had modified the mode of virus replication. It is believed that renewed virus replication, after the cessation of an antiretroviral therapy, begins from integrated or unintegrated proviral DNA.

Therefore, this DNA is an important biomarker of the HIV reservoir which persists after antiretroviral therapy, which opens the way for new types of treatment aimed at eradicating the infection.

Example 4 Location of the Active DNA in Blood Fractions

The heparinized blood of several HIV+ART-treated patients was run on a Ficoll gradient. DNA was extracted from the three main fractions: plasma (with platelets), white cells layer and the erythrocyte pellet. Each DNA extract was tested for EMS emission.

In all the patients with undetectable virus load, only the DNA from the plasma and the erythrocyte fractions gave strongly positive signals. The white cell layer-derived DNA gave no signal or weak signals. In ART-treated patients with remaining high virus load, only the plasma-derived DNA was positive.

Fractionation on Ficoll Gradient

Peripheral whole blood from patients was collected in vacutainer tubes containing lithium heparin. 3 ml of whole blood were diluted with 10 ml phosphate buffered saline (PBS) buffer and layered over 3 ml of Ficoll-paque (1.077 g/ml density; Amersham Biosciences) in 15 ml Leucosep® tubes and centrifuged at 1000×g for 10 min at 4° C. Plasma was removed; the red blood cell (RBC) pellet and the white blood cells (WBC) were washed 2 times with 10 ml of PBS and centrifuged at 250×g.

DNA Extraction

Plasma DNA, WBC DNA and RBC DNA were extracted by Proteinase K in the presence of SDS (sodium dodecyl sulfate) and further deproteinized by phenol-chloroform mixture. The pellet obtained by ethanol precipitation was resuspended in Tris 10⁻² M, pH 7.6 and an aliquot was diluted 1/100 in water. The dilution (10⁻²) was filtered first through a 450 nM filter and the resulting filtrate was then filtered again on a 20 nM filter Anotop (Whatman). The filtrate was further diluted in serial decimal (i.e., 1:9) dilutions in water.

Detection of EMS

The filtrates from plasma were analyzed just after filtration for production of electromagnetic waves of low frequency. A device was used that been previously designed by Benveniste and Coll (1996; 2003)(incorporated by reference) for the detection of signals produced by isolated molecules endowed with biological activity. Briefly, 100 nM or 20 nM filtrates are serially diluted 1 in 10 (0.1+0.9 in sterile water (medical grade). The first 2 dilutions (1/10 and 1/100) were done in serum-free RPMI medium, in order to avoid eventual protein precipitation in deionized water. Each dilution was done in 1.5 mL Eppendorf plastic tubes, which are then tightly stoppered and strongly agitated on a Vortex apparatus for at least 2 seconds, up to 15 seconds or more. This step has been found important for the generation of signals. After all dilutions have been made (generally 15-20, 1:10 dilutions), the stoppered tubes were read one by one.

EMS Measurement

To capture and analyze the EMS, a coil, bobbin of copper wire, was used and connected to a Sound Blaster Card itself connected to a laptop computer, preferentially powered by its 12 volt battery. Each emission was recorded twice for 6 seconds, amplified 500 times and processed with different softwares for visualization of the signals on the computer's screen. The main harmonics of the complex signals were analyzed by utilizing several softwares of Fourier transformation.

In each experiment, the internal noise generated by the different pieces of the reading system was first recorded (coil alone, coil with a tube filled with water). Fourier analysis shows that the noise was predominantly composed of very low frequencies, probably generated at least in part by the 50/60 Hz ambient electric current. The use of the 12 V battery for the computer power supply did reduce, but not abolish this noise, which was found to be necessary for the induction of the resonance signals from the specific nanostructures. When dilutions of the HIV virus filtrate were recorded for wave emission, the first obvious phenomenon observed was an increase of the overall amplitude of the signals at certain dilutions over the background noise and also an increase in frequencies. This change was abolished if the tube to be analyzed was placed inside a box sheltered with sheets of copper and mumetal. Fourier analysis of the HIV virus signals showed a shift towards higher frequencies close to 1000 Hz and multiples of it. Profiles were identical for all the dilutions showing an increase in amplitude. The first low dilutions were usually negative, showing the background noise only. Positive signals were usually obtained at dilutions ranging from 10⁻⁵ to 10⁻⁸ or 10⁻¹². Higher dilutions were again negative. The positive dilutions varied according to the type of filtration, the 20 nM filtrate being generally positive at dilutions higher than those of the 100 nM filtrate. The original unfiltered suspension was negative at all dilutions, a phenomenon observed for all preparations analyzed.

Nature of the HIV Sequences at the Origin of EMS

It was determined from previous experiments that a single gene or even a fragment of a gene was sufficient to produce the EMS. Therefore an infectious DNA clone of HIV was used to test for EMS. The infectious DNA clone of HIV had been previously constructed from HIV LAI to determine which part of the viral genome was at the origin of EMS. To this end, some specific primers were designed for sorting out the main sequences corresponding to the different structural and regulatory genes of HIV, including LTR, Pol, Gag, Env, Nef, and Vif.

The amplicons and secondary amplicons resulting from nested-PCR were analyzed by agarose gel electrophoresis and yielded the expected fragment sizes. The DNA bands were extracted and purified, and assayed for EMS production at different dilutions. As a control, the entire HIV DNA genome isolated from a plasma was also tested and found positive for EMS. Several sequences (LTR, Nef and Env) were found to be a source of EMS.

The same primers were used to detect specific sequences in the DNA extracted from the plasma or the red blood cell pellet of the positive patients. The amplified LTR DNA fragment, visualized as a band of 104 by nested PCR, was constantly found in all preparations, followed infrequently by Nef and Env amplified fragments. Sequencing of the LTR band confirmed its HIV origin with 99% identity with the prototype HIV DNA (2 nucleotide differences out of 104). Interestingly, a higher sensitivity of detection was obtained by the use of reverse transcriptase (RT) before the use of the Taq polymerase in the PCR reaction.

However this reaction was not affected by prior RNAse treatment, indicating that a DNA template, not RNA, was also used by the RT enzyme.

In addition when aqueous dilutions were tested, a 10 to 100 time increase (1 to 2 decimal dilutions) of sensitivity was obtained, when each dilution was strongly agitated by vortex, as done for the detection of EMS.

Example 5 Increased Sensitivity of PCR

A method was developed that increases the sensitivity of PCR by 10 to 100 times over the current PCR technique, A sample containing DNA is filtered, and then serially diluted by 1/10 [1 part sample to 9 parts diluent] at each step in the dilution cycle which includes vigorous vortexing of the current dilution before proceeding to the next dilution in the series.

Another aspect of the method, which can be combined with the vigorous vortexing of the sample, is to treat the sample with an RNase. The sample is first filtered and then treated with an RNase. After the RNase treatment, the sample is processed as described above with serial dilutions (1 part sample to 9 parts diluent) with vigorous vortexing between each serial dilution.

Vigorous vortexing is defined as more than mere the quick vortexing done with samples in a laboratory. The vortexing should be sustained for several seconds to ten's of seconds. Samples in the experiments were routinely vortexed for 15 seconds or more, and this vortexing was repeated after each dilution. The vigorous vortexing of the diluted sample is important in obtaining the increased sensitivity. The vigorous vortexing of the sample is believed to cause the DNA to induce nanostructures. The samples can be analyzed with PCR, nested-PCR, RT-PCR, or nested-RT-PCR.

PCR Primers

PCR primer sequences were retrieved from the online Primer Bank data base, These primers were synthesized at the Molecular Biology Core Facility, Massachusetts General Hospital. Both UV absorbance and capillary electrophoresis were used to assess the quality of primer synthesis.

One-step reverse transcriptase (RT)-PCR experiments were performed with the Mastercycler® ep (Eppendorf). A 50 μl RT reaction included 25 μl of 2xRT-PCR buffer, 16.6 μl of nuclease-free-water, 0.4 μl of 25 mM of each deoxynucleoside triphosphate (dNTPs), 1 μl of 50 μM of each appropriate primer (Invitrogen), 1-4 ng/ml of total DNA and 1 μl of iScript RT (BioRad). The RT-PCR mixtures were pre-heated at 42° C. for 30 minutes (RT step) followed by 1 cycle (inactivation and denaturation step) at 95° C. for 3 minutes, followed by 42 PCR cycles of amplification (95° C. for 30 seconds; 56° C. for 30 seconds; 78° C. for 2 minutes). A final extension step was performed at 78° C. for 10 minutes.

The PCR mixture (50/41) contained 29.4 μl of nuclease-water-free, 5 μl of 10× Taq PCR buffer, 8 μl of 25 mM MgCl₂, 0.4 μd of 25 mM dNTPs, 1 μl of 50 μM of each appropriate primer, 5 μl of RT-PCR product and 1 μl of 5 U/μl Taq DNA polymerase (Invitrogen).

The PCR was performed with the Mastercycler® ep (Eppendorf). The PCR mixtures were pre-heated at 95° C. for 3 minutes (inactivation and denaturation step), followed by 42 PCR cycles of amplification (95° C. for 30 seconds; 56° C. for 30 seconds; 78° C. for 2 minutes). A final extension step was performed at 78° C. for 10 minutes.

Specific internal primers were used for the second round of amplification [nested-PCR].

The amplification products were separated on a 1.2% Agarose gel electrophoresis/EtBr gel and visualized using a Molecular Imager® Gel Doc™ XR System (BioRad).

Infected CEM Cells

In vitro experiments were set up in which CEM cells were infected with a prototype HIV-1 strain, HIV LAI. Prior to the experiments, cells and infecting virus were first checked for mycoplasma contamination by using a highly sensitive PCR technology based on 16s ribosomal RNA. Traces of Mycoplasma arginini were found only in control CEM cells, but no electromagnetic signals (EMS) could be detected in the culture supernatant of such cells.

By contrast, EMS was detected in dilutions of the culture supernatant of the HIV-infected cells, when the cytopathic effect was obvious. Filtration through 20 nM filters was found to be necessary to detect the EMS, indicating that the source of the EMS was smaller than this size and therefore smaller than the intact virus particles whose diameter range between 100 to 120 nM.

The density of such particles was evaluated by centrifuging to equilibrium an aliquot of the infected CEM supernatant on a sucrose density gradient with conditions where HIV virions form a sharp band at the density of 1.16.

By contrast, the nanoparticles producing the EMS were associated with fractions ranging in densities from 1.15 to 1.25. A longer time of centrifugation used to improve the density equilibrium did not modify this profile.

Analysis of Dilutions Versus EMS Emission

The lower dilutions, which logically should contain a larger number of signal-producing structures, were “silent”. When 0.1 mL of a negative low dilution (e.g. 10⁻³) was added to 0.4 mL or 0.9 mL of a positive dilution (10⁻⁸), the latter became negative. This indicated that the “silent” low dilutions were self-inhibitory, probably by interference of the multiple sources emitting in the same wave length or slightly out of phase, like a radio jamming. Alternatively, the abundance of nanostructures can form a gel in water and therefore are prevented to vibrate.

Influence of Order of Reading Samples to Emitting EMS

The results were independent of the order in which the samples were read, whether in descending dilutions from to the lowest to the highest or in ascending dilutions from the highest to the lowest. Diluted samples placed in a random order (labels unknown to the person reading the samples) indicated the same range of positive dilutions was detected, if each tube was well separated from the other, to avoid their “cross talk”. The results also were independent of the location of the reading site. Even though the background noise was variable, according to the location and time of recording (generally higher in large cities than in isolated areas), positive signals were always clearly differentiated over the background by higher frequency peaks.

Nature of the Aqueous Nanostructures:

Treatments by RNAse A (Promega, 1 μg/ml, 37° C., 1 hour), DNase I (Invitrogen, 10 U/μg DNA, 37° C., 18 hours), Lysozyme (Fisher, 1 mg/mL, 37° C., 10 minutes), Proteinase K (Promega, 0.12 mg/mL, in 1% sodium dodecyl sulphate, 56° C., 1 hour) did not suppress the EMS producing activity of the “loud” dilutions nor did activate the “silent” dilutions. However, heating at 70° C. for 30 minutes suppressed irreversibly the activity, as well as did freezing for 1 hour at −20° C. or −60° C. DMSO (10%), and formamide (10%) had no effect. Treatment with lithium cations, known to affect the hydrogen bonding of water molecules, was able to reduce the intensity of the signals, while the range of the positive dilutions remained unchanged.

Nature of the Origin of the Nanostructures:

In preliminary experiments, it had been observed that a pretreatment of a suspension of bacteria did not alter its capacity to induce the electromagnetic signals, even though it killed the virus. This treatment degraded the viral RNA without attacking double-helical DNA. This suggested that the source of the signals may be the DNA itself. Likewise, DNA extracted from HIV infected samples by the classical phenol: chloroform technique was able upon filtration and appropriate dilutions in water to emit EMS similar to those produced by HIV virus under the same conditions. DNAse treatment of the extracted DNA solution abolishes its capacity to emit signals, at the condition that the nanostructures previously induced by the DNA are destroyed.

Sample Analysis

A sample was treated by Proteinase K in the presence of SDS (sodium dodecyl sulfate) and further deproteinized by phenol-chloroform mixture. The pellet obtained by ethanol precipitation was resuspended in Tris 10⁻² M, pH 7.6 and an aliquot was diluted 1/100 in water. The dilution (10⁻²) was filtered first through a 450 nM filter and the resulting filtrate was then filtered again on a 20 nM filter. The filtrate was further diluted in serial 1:10 dilutions in water as previously described. As for the intact microorganisms, the filtration step was found to be essential for detection of the EMS in the DNA dilutions. In its absence, no signals could be detected at any dilutions. In contrast to the HIV viral suspension, where the filtration was supposed to retain DNA, the filtration at 20 nM did not retain the DNA, which was still present in the filtrate, as measured by optical density. In the case of DNA, the role of the 20 nM filtration is probably to dissociate the network of nanostructures organized in a gel-like liquid crystal at high concentrations in water, allowing their dispersion in further dilutions. The dilutions positive for EMS were in the same range that those observed for the viral suspensions, generally between 10⁻⁷ to 10⁻¹³.

DNA Content of Dilutions

At the high dilution of 10⁻⁹, calculations indicated that there is no DNA molecule of MW larger than 10⁵ in the solution, making it unlikely that the EMS were produced directly by the DNA itself, but rather by the self-sustained nanostructures induced by the DNA. Further demonstration that the EMS produced came from DNA was shown by their disappearance after DNAse treatment. This inactivation was however only complete when the nanostructures induced in the DNA solution which were themselves resistant to DNAse were previously fully destroyed. This destruction was obtained either by freezing the DNA solution at −20° C. for 1 hour or heating it at 90° C. for 30 minutes. After slow cooling to allow the heated DNA to reanneal, DNAse 1 at a final concentration of 10 U/μg of DNA was added and the mixture was incubated at 37° C. for 18 hours in the presence of 5 mM of MgCl₂. An aliquot of the untreated DNA solution was kept as a positive control. The DNAse-treated preparation was found completely devoid of EMS emission at any dilution. Treatment of the DNA solution by a restriction enzyme acting at many sites did not suppress the production of EMS, suggesting that this emission was linked to rather short sequences or was associated with rare sequences.

Nature of the DNA Sequences at the Origin of the EMS:

It is believed that the DNA able to generate EMS suggests that this DNA is associated with pathogenicity in humans and other animals. By contrast, good viruses are probably negative for EMS emission. This suggested that only some sequences of DNA were at the origin of the EMS, since pathogenicity was often associated with the capacity of the microorganism to bind eukaryotic cells, particularly mucosal cells. The disclosed methods and compositions can be used either manually by a technician in a laboratory or can be combined into an automatic analyzer of blood, body fluids, tissue and cells from people and, animals in general, to detect a reservoir virus infection in subjects not showing a viral load. Automatic analysis of samples from people by a laboratory machine is contemplated by this disclosure. Additionally, it is contemplated that a scanner could be used to detect a viral infection by a non-invasive technique (e.g., placing a palm or finger on a scanner plate) to detect the EMS emitted by an EMS generating entity associated with that disease. Although, the HIV virus has been used to demonstrate the production of EMS from pathogenic viruses, other pathogenic viruses (e.g., influences and HPV) can be used with the disclosed methods, compositions and apparatuses. Other embodiments of the invention include:

A method for detecting electromagnetic waves derived from a polynucleotide, such as viral DNA, comprising: extracting and purifying nucleic acids from a sample; diluting the extracted purified nucleic acids in an aqueous solvent; measuring a low frequency electromagnetic emission over time from the diluted extracted purified nucleic acids in an aqueous solvent; performing a signal analysis of the low frequency electromagnetic emission over time; and producing an output, based on the signal analysis, in dependence on the DNA in the sample. Advantageously, this method involves one in which the output varies in dependence on DNA in the sample derived from a pathogenic virus in plasma of a patient suffering from a chronic disease. The viral DNA may be extracted from a biological or physiological sample including tissue, cells, blood, feces, urine, saliva, tears, seminal fluid, sweat, vaginal fluids of a subject, particularly of a subject having or suspected of having a viral infection. The sample may also be extracted from a source external to the subject such as from food or portable water or from an environmental sample. In some embodiments, a DNA sample will be extracted from a sample that has been previously stored, lyophilized, or frozen and stored at a temperature between about −20° C. and −70° C.

The polynucleotide, preferably DNA, may be extracted and purified by diluting the sample with an aqueous buffer and mixing; degrading protein in the diluted sample; precipitating DNA from the buffer solution; and resuspending the precipitated DNA in an aqueous solution. This method may further comprise filtering the resuspended DNA through at least one submicron filter, wherein the sample measured comprises the filtrate. The sample may be made by diluting the filtrate in an aqueous solution prior to measuring, for example, dilution of a resuspended DNA to a concentration of 10⁻² to 10⁻²⁰ of its concentration prior to measurement.

The measuring in such a method may comprise placing the diluted extracted purified nucleic acids near an antenna adapted to receive electromagnetic signals having a frequency approaching about 0 Hz, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 kHz and up to and including 20 kHz, and receiving the electromagnetic signals from the antenna.

The method as described above may employ signal analysis which comprises performing a time domain to frequency domain transformation on the signal and which may further comprise comparing frequency domain transformed signals from two different samples. Signal analysis may also be performed by applying a threshold function to the frequency domain transformed signal. Such signal analysis can be implemented on a general purpose computer and said output is presented through a graphic user interface. Signal analysis may comprise, be determined as, or be displayed as three-dimensional histogram. Background noise reduction may be performed as a part of the signal analysis.

The signal analysis preferably comprises analysis of signal components having frequencies between about 1-20,000 Hz and/or employs a diluting step which dilutes the polynucleotide, such as DNA, to about 10⁻⁷ to 10⁻¹³ of its original concentration.

Another embodiment of the invention is a composition comprising a filtered, vortexed, diluted sample of a polynucleotide, such as DNA, preferably DNA from a pathogenic virus such as a HIV, wherein the filtered, vortexed, diluted sample of DNA has a detectable electromagnetic signal.

The invention also involves a method of detecting an animal having an infection by a pathogen or pathogenic particle comprising placing a body part of an animals on an EMS detecting device, measuring the EMS from the body part, analyzing the EMS, and determining if the EMS corresponds to an EMS produced by a pathogenic particle.

Alternatively, a pathogenic infection in an animal may be detected by a process comprising a) obtaining a body fluid from an animal, b) filtering the body fluid to obtain a filtered body fluid, c) vortexing the filtered body fluid, d) diluting the filtered body fluid in step b) by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, e) measuring an EMS from the diluted body fluid in step d), f) analyzing the EMS, and g) determining if the EMS corresponds to an EMS produced by a pathogenic particle. In such a method steps c) and d) are repeated at least one time, twice or more than two times.

The invention in another aspect involves a method of detecting an animal with a pathogenic infection comprising the steps of: a) obtaining a body fluid from an animal, b) filtering the body fluid to obtain a filtered body fluid, c) serial diluting of the filtered body fluid until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9; d) measuring an EMS from the diluted body fluid in step d), e) analyzing the EMS, f) determining if the EMS corresponds to an EMS produced by a pathogenic particle.

A method of detecting an animal with a pathogenic infection comprising the steps of: a) obtaining a sample of tissue from an animal, b) extracting DNA from the sample of tissue from the animal, c) making a solution containing the DNA from the sample of tissue from the animal, d) filtering the solution in step c), e) vortexing the solution in step d), f) diluting the solution in step e) by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, g) measuring an EMS from the solution in step f), h) analyzing the EMS, and i) determining if the EMS corresponds to an EMS produced by a pathogenic particle. This method may comprise repeating steps e) and f) at least one time or more than twice.

A method of detecting an animal with a pathogenic infection comprising the steps of: a) obtaining a sample of tissue from an animal, b) extracting DNA from the sample of tissue from the animal, c) making a solution containing the DNA from the sample of tissue from the animal, d) filtering the solution in step c), e) serial diluting of the solution in step c) wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9; g) measuring an EMS from the solution in step f), h) analyzing the EMS, and i) determining if the EMS corresponds to an EMS produced by a pathogenic particle.

A method of detecting an animal with a pathogenic infection comprising the steps of: a) obtaining a sample of cells from an animal, b) extracting DNA from the sample of cells from the animal, c) making a solution containing the DNA from the sample of cells from the animal, d) filtering the solution in step c), e) vortexing the solution in step d), f) diluting the solution in step e) by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, g) measuring an EMS from the solution in step 0, h) analyzing the EMS, i) determining if the EMS corresponds to an EMS produced by a pathogenic particle. This method may comprise repeating steps e) and f) at least one time, twice or more than twice.

A method of detecting an animal with a pathogenic infection comprising the steps of: a) obtaining a sample of cells from an animal, b) extracting DNA from the sample of cells from the animal, c) making a solution containing the DNA from the sample of cells from the animal, d) filtering the solution in step c), e) serial diluting of the solution in step d) wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9; f) measuring an EMS from the solution in step f), g) analyzing the EMS, and h) determining if the EMS corresponds to an EMS produced by a pathogenic particle.

A method of increasing the sensitivity of PCR comprising the steps of: a) obtaining a sample to be analyzed by PCR, b) filtering the sample, c) vortexing the sample, d) diluting the sample by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, e) analyzing diluted sample with PCR. This method may involve repeating steps c) and d) at least one time or more than twice.

A method of increasing the sensitivity of PCR comprising the steps of: a) obtaining a sample to be analyzed by PCR, b) filtering the sample, c) serial diluting of the sample until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid at a dilution of at least 1:9, preferably diluting the sample at a dilution of 1:9; d) diluting the sample at a dilution of at least 1:9, preferably diluting the sample at a dilution of 1:9, and e) analyzing diluted sample with PCR.

A method to increase the sensitivity of PCR to detect HIV comprising: obtaining a sample from an animal, filtering the sample, treating filtered sample with an RNase, vortexing the sample, diluting the sample by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, and analyzing diluted sample with PCR, nested-PCR, RT-PCR, or nested-RT-PCR; or combinations thereof. The steps of vortexing and diluting may be performed once, twice or repeated more than twice.

A method to increase the sensitivity of PCR to detect HIV comprising: obtaining a sample from an animal, filtering the sample, treating the filtered sample with an RNAse; serial diluting of the filtered sample body fluid until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9; vortexing the sample, diluting the sample by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, and analyzing diluted sample with PCR, nested-PCR, RT-PCR, or nested-RT-PCR; or combinations thereof.

A method to detect reservoir HIV virus comprising: obtaining a sample of body fluid from an animal, filtering the sample, vortexing the sample, diluting the sample at a dilution of at least 1:9, preferably diluting the sample at a dilution of 1:9, measuring an EMS from the diluted sample, analyzing the EMS, and determining if the EMS corresponds to HIV virus. The steps of vortexing and diluting may be performed once, twice or repeated more than twice.

A method to detect reservoir HIV virus comprising: obtaining a sample of body fluid from an animal, filtering the sample, serial diluting of the sample until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9; measuring an EMS from the diluted sample, analyzing the EMS, and determining if the EMS corresponds to HIV virus.

A method to detect reservoir HIV virus comprising: obtaining a sample of body fluid from an animal, filtering the sample, treating filtered sample with an RNase, vortexing the sample, diluting the sample by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, and analyzing diluted sample with RT-PCR. The steps of vortexing and diluting may be performed once, twice or repeated more than twice.

A method to detect reservoir HIV virus comprising: obtaining a sample of body fluid from an animal, filtering the sample, treating filtered sample with an RNAse, serial diluting of the filtered sample with the RNase body until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9; analyzing diluted sample with RT-PCR.

A method to detect reservoir HIV virus comprising: obtaining a sample of body fluid from an animal, filtering the sample, treating filtered sample vortexing the sample, diluting the sample by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, and analyzing diluted sample using HIV primers with nested PCR. In this method the steps of vortexing and diluting can be performed once, twice or are repeated more than twice.

A method to detect reservoir HIV virus comprising: obtaining a sample of body fluid from an animal, filtering the sample, treating filtered sample, serial diluting of the filtered sample until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid at a dilution of at least 1:9, preferably diluting the sample at a dilution of 1:9; analyzing diluted sample using HIV primers with nested PCR.

An apparatus to analyze a sample for a pathogenic infection comprising: a sample loading device; a sample filtering device; a sample diluting device; a sample vortexing device; a sample measuring device for EMS; an EMS analyzer; and a data display device.

An apparatus to analyze an animal for a pathogenic infection comprising: a surface for placing a body part; a measuring device for EMS; an EMS analyzer; and a data display device.

An apparatus to analyze cells for a pathogenic infection comprising: a sample loading device; a sample DNA extraction device; a sample filtering device; a sample diluting device; a sample vortexing device; a sample measuring device for EMS; an EMS analyzer; and a data display device.

In another aspect, the invention encompasses an apparatus to analyze a tissue for a pathogenic infection comprising: a sample loading device; a sample DNA extraction device; a sample filtering device; a sample diluting device; a sample vortexing device; a sample measuring device for EMS; an EMS analyzer; and a data display device.

Another facet of the invention is a method to determine efficiency of a treatment for a pathogenic infection in a person comprising: measuring an EMS in a person corresponding to an EMS from a pathogenic particle; treating the person with a treatment for which an efficiency is being determined; measuring an EMS in the person treated with the treatment; and determining the relationship between the EMS before treatment and the EMS after treatment.

Yet another aspect of the invention is a method to determine a cure of an HIV infection in a person comprising: measuring an EMS in a person corresponding to an EMS from a HIV virus; treating the person with a treatment for which a cure is expected; and not detecting an EMS in the person corresponding to the EMS from the HIV virus.

The invention also relates to a method to treat HIV comprising: measuring an EMS in a person corresponding to an EMS-generating particle from a HIV virus; treating the person with a treatment to eliminate the EMS-generating particle from the HIV virus.

A method to decrease an EMS from an EMS emitting sample comprising: placing an EMS emitting sample next to a non-EMS emitting sample, and waiting an effective amount of time to decrease the EMS in the EMS emitting sample.

A method to induce an EMS in a sample not emitting an EMS comprising: placing an EMS emitting sample next to a vial containing a fluid, and waiting an effective amount of time to induce an EMS in the vial containing the fluid.

A method of detecting viral DNA in a patient with undetectable viral RNA comprising: obtaining a sample of body fluid from a patient, filtering the sample, treating filtered sample with an RNase, vortexing the sample, diluting the sample by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, and analyzing diluted sample with RT-PCR. This method may employ the steps of vortexing and diluting being performed once, twice, or more than twice.

A method of detecting viral DNA in a patient with undetectable viral RNA comprising: obtaining a sample of body fluid from a patient, filtering the sample, treating filtered sample with an RNase, serial diluting of the filtered sample with the RNase until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid at a dilution of a least 1:9, preferably diluting the sample at a dilution of 1:9; and analyzing diluted sample with RT-PCR.

A method to assess the eradication of a viral infection by reduction of viral DNA comprising: measuring an EMS in a person corresponding to an EMS from a viral DNA; treating the person with a treatment for which an efficiency is being determined; measuring an EMS in the person treated with the treatment; and determining the relationship between the EMS before treatment and the EMS after treatment. This method may be applied to subjects having viral infections caused by a HIV, Influenza virus, or other viruses, especially persistent viruses.

A method to confirm or detect an EMS generated by a HIV virus comprising: obtaining a sample from a patient, filtering the sample, treating filtered sample with an RNase, vortexing the sample, diluting the sample by a factor of at least 1:9, preferably diluting the sample at a dilution of 1:9, and amplifying the diluted sample with RT-PCR using a PCR primer for a HIV gene sequence. This method may be performed using a PCR primer for LTR, Gag, Env, Tat, Rev, Nef, Vif, Vpr, Vpu, Pol, and/or for double LTR.

The invention also is directed to a method to confirm or detect an EMS generated by a HIV virus comprising: obtaining a sample from a patient, filtering the sample, treating filtered sample with an RNase, serial diluting of the filtered sample with the RNase until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid by a factor or at least 1:9, preferably diluting the sample at a dilution of 1:9; and amplifying the diluted sample with RT-PCR using a PCR primer for a HIV gene sequence. This method also includes one in which the PCR primer is for LTR, Gag, Env, Tat, Rev, Nef, Vif, Vpr, Vpu, Pol and/or is for double LTR.

Additional Embodiments of the Disclosed Method Time Period of Vortexing

In one embodiment of the disclosed method the time period of vortexing is at least 1 second. In one embodiment of the disclosed method the time period of vortexing is at least 2 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 3 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 4 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 5 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 6 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 7 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 8 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 9 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 10 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 11 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 12 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 13 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 14 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 15 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 16 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 17 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 18 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 19 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 20 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 25 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 30 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 35 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 40 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 45 seconds. In one embodiment of the disclosed method the time period of vortexing is at east 50 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 55 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 60 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 90 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 120 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 150 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 180 seconds. In one embodiment of the disclosed method the time period of vortexing is at least 5 minutes. In one embodiment of the disclosed method the time period of vortexing is at least 10 minutes.

In one embodiment of the disclosed method the time period of vortexing is more than 1 second. In one embodiment of the disclosed method the time period of vortexing is more than 2 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 3 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 4 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 5 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 6 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 7 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 8 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 9 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 10 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 11 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 12 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 13 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 14 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 15 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 16 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 17 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 18 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 19 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 20 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 25 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 30 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 35 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 40 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 45 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 50 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 55 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 60 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 90 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 120 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 150 seconds, In one embodiment of the disclosed method the time period of vortexing is more than 180 seconds. In one embodiment of the disclosed method the time period of vortexing is more than 5 minutes. In one embodiment of the disclosed method the time period of vortexing is more than 10 minutes.

In one embodiment of the disclosed method the time period of vortexing is approximately 1 second. In one embodiment of the disclosed method the time period of vortexing is approximately 2 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 3 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 4 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 5 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 6 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 7 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 8 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 9 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 10 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 11 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 12 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 13 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 14 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 15 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 16 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 17 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 18 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 19 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 20 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 25 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 30 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 35 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 40 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 45 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 50 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 55 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 60 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 90 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 120 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 150 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 180 seconds. In one embodiment of the disclosed method the time period of vortexing is approximately 5 minutes. In one embodiment of the disclosed method the time period of vortexing is approximately 10 minutes.

In one embodiment of the disclosed method the time period of vortexing is 1 second. In one embodiment of the disclosed method the time period of vortexing is 2 seconds. In one embodiment of the disclosed method the time period of vortexing is 3 seconds. In one embodiment of the disclosed method the time period of vortexing is 4 seconds. In one embodiment of the disclosed method the time period of vortexing is 5 seconds. In one embodiment of the disclosed method the time period of vortexing is 6 seconds. In one embodiment of the disclosed method the time period of vortexing is 7 seconds. In one embodiment of the disclosed method the time period of vortexing is 8 seconds. In one embodiment of the disclosed method the time period of vortexing is 9 seconds. In one embodiment of the disclosed method the time period of vortexing is 10 seconds. In one embodiment of the disclosed method the time period of vortexing is 11 seconds. In one embodiment of the disclosed method the time period of vortexing is 12 seconds. In one embodiment of the disclosed method the time period of vortexing is 13 seconds. In one embodiment of the disclosed method the time period of vortexing is 14 seconds. In one embodiment of the disclosed method the time period of vortexing is 15 seconds. In one embodiment of the disclosed method the time period of vortexing is 16 seconds. In one embodiment of the disclosed method the time period of vortexing is 17 seconds. In one embodiment of the disclosed method the time period of vortexing is 18 seconds. In one embodiment of the disclosed method the time period of vortexing is 19 seconds. In one embodiment of the disclosed method the time period of vortexing is 20 seconds. In one embodiment of the disclosed method the time period of vortexing is 25 seconds. In one embodiment of the disclosed method the time period of vortexing is 30 seconds. In one embodiment of the disclosed method the time period of vortexing is 35 seconds. In one embodiment of the disclosed method the time period of vortexing is 40 seconds. In one embodiment of the disclosed method the time period of vortexing is 45 seconds. In one embodiment of the disclosed method the time period of vortexing is 50 seconds. In one embodiment of the disclosed method the time period of vortexing is 55 seconds. In one embodiment of the disclosed method the time period of vortexing is 60 seconds. In one embodiment of the disclosed method the time period of vortexing is 90 seconds. In one embodiment of the disclosed method the time period of vortexing is 120 seconds. In one embodiment of the disclosed method the time period of vortexing is 150 seconds. In one embodiment of the disclosed method the time period of vortexing is 180 seconds. In one embodiment of the disclosed method the time period of vortexing is 5 minutes. In one embodiment of the disclosed method the time period of vortexing is 10 minutes.

Filtration, Dilution and Vortex Steps

In one embodiment of the disclosed method the sample is diluted. In one embodiment of the disclosed method the sample is serially diluted. In one embodiment of the disclosed method the sample is diluted in series with the same dilution factor. In one embodiment of the disclosed method the sample is diluted in series with different dilution factors. In one embodiment of the disclosed method the sample is diluted and then vortexed. In one embodiment of the disclosed method the sample is vortexed and then diluted.

In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated twice. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated three (3) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated four (4) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated five (5) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated six (6) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated seven (7) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated eight (8) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated nine (9) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated ten (10) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated eleven (11) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated twelve (12) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least twice. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least three (3) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least four (4) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least five (5) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least six (6) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least seven (7) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least eight (8) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least nine (9) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least ten (10) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least eleven (11) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least twelve (12) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated at least twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than twice. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than three (3) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than four (4) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than five (5) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than six (6) times.

In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than seven (7) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than eight (8) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than nine (9) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than ten (10) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than eleven (11) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than twelve (12) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and these steps are repeated more than twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated twice. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated three (3) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated four (4) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated five (5) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated six (6) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated seven (7) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated eight (8) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated nine (9) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated ten (10) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated eleven (11) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated twelve (12) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least twice. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least three (3) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least four (4) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least five (5) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least six (6) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least seven (7) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least eight (8) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least nine (9) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least ten (10) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least eleven (11) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least twelve (12) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than twice. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than three (3) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than four (4) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than five (5) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than six (6) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than seven (7) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than eight (8) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than nine (9) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than ten (10) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than eleven (11) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than twelve (12) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than twenty (20) times.

Filtration, Vortex and Dilution Steps

In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated twice. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated three (3) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated four (4) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated five (5) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated Six (6) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated seven (7) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated eight (8) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated nine (9) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated ten (10) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated eleven (11) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated twelve (12) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least twice. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least three (3) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least four (4) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least five (5) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least six (6) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least seven (7) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least eight (8) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least nine (9) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least ten (10) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least eleven (11) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least twelve (12) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated at least twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than twice. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than three (3) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than four (4) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than five (5) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than six (6) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than seven (7) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than eight (8) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than nine (9) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than ten (10) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than eleven (11) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than twelve (12) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than thirteen (13) times. In one embodiment of the disclosed method the. sample is filtered, vortexed and diluted, and these steps are repeated more than fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and these steps are repeated more than nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the se steps are repeated more than twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated twice. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated three (3) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated four (4) tines. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated five (5) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated six (6) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated seven (7) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated eight (8) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated nine (9) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated ten (10) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated eleven (11) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated twelve (12) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least twice. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least three (3) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least four (4) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least five (5) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least six (6) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least seven (7) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least eight (8) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least nine (9) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least ten (10) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least eleven (11) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least twelve (12) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least twenty (20) times.

In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than twice. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than three (3) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than four (4) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than five (5) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than six (6) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than seven (7) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than eight (8) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than nine (9) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than ten (10) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than eleven (11) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than twelve (12) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than thirteen (13) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than fourteen (14) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than fifteen (15) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than sixteen (16) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than seventeen (17) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than eighteen (18) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than nineteen (19) times. In one embodiment of the disclosed method the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than twenty (20) times.

Dilution Step

In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻². In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻³. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻⁴. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻⁵. In one, embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻⁶. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻⁷. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻⁸. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻⁹. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹⁰. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹¹. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹². In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹³. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹⁴. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹⁵. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹⁶. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹⁷. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹⁸. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻¹⁹. In one embodiment of the disclosed method the EMS is measured in a sample diluted 10⁻²⁰.

In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻². In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻³. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻⁴. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻⁵. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻⁶. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻⁷. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻⁸. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻⁹. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹⁰. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹¹. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹². In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹³. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹⁴. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹⁵. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹⁶. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹⁷. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹⁸. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻¹⁹. In one embodiment of the disclosed method the EMS is measured in a sample diluted at least 10⁻²⁰.

In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻². In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻³. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻⁴. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻⁵. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻⁶. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻⁷. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻⁸. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻⁹. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹⁰. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹¹. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹². In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹³. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹⁴. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹⁵. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹⁶. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹⁷. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹⁸. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻¹⁹. In one embodiment of the disclosed method the EMS is measured in a sample diluted more than 10⁻²⁰.

In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻². In one embodiment of the disclosed method the EMS measured in a sample diluted approximately 10⁻³. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻⁴. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻⁵. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻⁶. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻⁷. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻⁸. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻⁹. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹⁰. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹¹. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹². In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹³. In one embodiment of the disclosed method the EMS is measured a sample diluted approximately 10⁻¹⁴. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹⁵. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹⁶. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹⁷. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹⁸. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻¹⁹. In one embodiment of the disclosed method the EMS is measured in a sample diluted approximately 10⁻²⁰.

Dilution Factor

In one embodiment of the disclosed method the dilution factor is 1:1. In one embodiment of the disclosed method the dilution factor is 1:2. In one embodiment of the disclosed method the dilution factor is 1:3. In one embodiment of the disclosed method the dilution factor is 1:4. In one embodiment of the disclosed method the dilution factor is 1:5. In one embodiment of the disclosed method the dilution factor is 1:6. In one embodiment of the disclosed method the dilution factor is 1:7. In one embodiment of the disclosed method the dilution factor is 1:8. In one embodiment of the disclosed method the dilution factor is 1:9, preferably diluting the sample at a dilution of 1:9. In one embodiment of the disclosed method the dilution factor is 1:10. In one embodiment of the disclosed method the dilution factor is 1:11. In one embodiment of the disclosed method the dilution factor is 1:12. In one embodiment of the disclosed method the dilution factor is 1:13. In one embodiment of the disclosed method the dilution factor is 1:14. In one embodiment of the disclosed method the dilution factor is 1:15. In one embodiment of the disclosed method the dilution factor is 1:16. In one embodiment of the disclosed method the dilution factor is 1:17. In one embodiment of the disclosed method the dilution factor is 1:18. In one embodiment of the disclosed method the dilution factor is 1:19. In one embodiment of the disclosed method the dilution factor is 1:20. In one embodiment of the disclosed method the dilution factor is 1:25. In one embodiment of the disclosed method the dilution factor is 1:30. In one embodiment of the disclosed method the dilution factor is 1:35. In one embodiment of the disclosed method the dilution factor is 1:40. In one embodiment of the disclosed method the dilution factor is 1:45. In one embodiment of the disclosed method the dilution factor is 1:50. In one embodiment of the disclosed method the dilution factor is 1:55. In one embodiment of the disclosed method the dilution factor is 1:60. In one embodiment of the disclosed method the dilution factor is 1:65. In one embodiment of the disclosed method the dilution factor is 1:70. In one embodiment of the disclosed method the dilution factor is 1:75. In one embodiment of the disclosed method the dilution factor is 1:80. In one embodiment of the disclosed method the dilution factor is 1:85. In one embodiment of the disclosed method the dilution factor is 1:90. In one embodiment of the disclosed method the dilution factor is 1:95. In one embodiment of the disclosed method the dilution factor is 1:100.

In one embodiment of the disclosed method the dilution factor is at least 1:1. In one embodiment of the disclosed method the dilution factor is at least 1:2. In one embodiment of the disclosed method the dilution factor is at least 1:3. In one embodiment of the disclosed method the dilution factor is at least 1:4. In one embodiment of the disclosed method the dilution factor is at least 1:5. In one embodiment of the disclosed method the dilution factor is at least 1:6. In one embodiment of the disclosed method the dilution factor is at least 1:7. In one embodiment of the disclosed method the dilution factor is at least 1:8. In one embodiment of the disclosed method the dilution factor is at least 1:9. In one embodiment of the disclosed method the dilution factor is at least 1:10. In one embodiment of the disclosed method the dilution factor is at least 1:11. In one embodiment of the disclosed method the dilution factor is at least 1:12. In one embodiment of the disclosed method the dilution factor is at least 1:13. In one embodiment of the disclosed method the dilution factor is at least 1:14. In one embodiment of the disclosed method the dilution factor is at least 1:15. In one embodiment of the disclosed method the dilution factor is at least 1:16. In one embodiment of the disclosed method the dilution factor is at least 1:17. In one embodiment of the disclosed method the dilution factor is at least 1:18. In one embodiment of the disclosed method the dilution factor is at least 1:19. In one embodiment of the disclosed method the dilution factor is at least 1:20. In one embodiment of the disclosed method the dilution factor is at least 1:25. In one embodiment of the disclosed method the dilution factor is at least 1:30. In one embodiment of the disclosed method the dilution factor is at least 1:35. In one embodiment of the disclosed method the dilution factor is at least 1:40. In one embodiment of the disclosed method the dilution factor is at least 1:45. In one embodiment of the disclosed method the dilution factor is at least 1:50. In one embodiment of the disclosed method the dilution factor is at least 1:55. In one embodiment of the disclosed method the dilution factor is at least 1:60. In one embodiment of the disclosed method the dilution factor is at least 1:65. In one embodiment of the disclosed method the dilution factor is at least 1:70. In one embodiment of the disclosed method the dilution factor is at least 1:75. In one embodiment of the disclosed method the dilution factor is at least 1:80. In one embodiment of the disclosed method the dilution factor is at least 1:85. In one embodiment of the disclosed method the dilution factor is at least 1:90. In one embodiment of the disclosed method the dilution factor is at least 1:95. In one embodiment of the disclosed method the dilution factor is at least 1:100.

In one embodiment of the disclosed method the dilution factor is more than 1:1. In one embodiment of the disclosed method the dilution factor is more than 1:2. In one embodiment of the disclosed method the dilution factor is more than 1:3. In one embodiment of the disclosed method the dilution factor is more than 1:4. In one embodiment of the disclosed method the dilution factor is more than 1:5. In one embodiment of the disclosed method the dilution factor is more than 1:6. In one embodiment of the disclosed method the dilution factor is more than 1:7. In one embodiment of the disclosed method the dilution factor is more than 1:8. In one embodiment of the disclosed method the dilution factor is more than 1:9. In one embodiment of the disclosed method the dilution factor is more than 1:10. In one embodiment of the disclosed method the dilution factor is more than 1:11. In one embodiment of the disclosed method the dilution factor is more than 1:12. In one embodiment of the disclosed method the dilution factor is more than 1:13. In one embodiment of the disclosed method the dilution factor is more than 1:14. In one embodiment of the disclosed method the dilution factor is more than 1:15. In one embodiment of the disclosed method the dilution factor is more than 1:16. In one embodiment of the disclosed method the dilution factor is more than 1:17. In one embodiment of the disclosed method the dilution factor is more than 1:18. In one embodiment of the disclosed method the dilution factor is more than 1:19. In one embodiment of the disclosed method the dilution factor is more than 1:20. In one embodiment of the disclosed method the dilution factor is more than 1:25. In one embodiment of the disclosed method the dilution factor is more than 1:30. In one embodiment of the disclosed method the dilution factor is more than 1:35. In one embodiment of the disclosed method the dilution factor is more than 1:40. In one embodiment of the disclosed method the dilution factor is more than 1:45. In one embodiment of the disclosed method the dilution factor is more than 1:50. In one embodiment of the disclosed method the dilution factor is more than 1:55. In one embodiment of the disclosed method the dilution factor is more than 1:60. In one embodiment of the disclosed method the dilution factor is more than 1:65. In one embodiment of the disclosed method the dilution factor is more than 1:70. In one embodiment of the disclosed method the dilution factor is more than 1:75. In one embodiment of the disclosed method the dilution factor is more than 1:80. In one embodiment of the disclosed method the dilution factor is more than 1:85. In one embodiment of the disclosed method the dilution factor is more than 1:90. In one embodiment of the disclosed method the dilution factor is more than 1:95. In one embodiment of the disclosed method the dilution factor is more than 1:100.

In one embodiment of the disclosed method the dilution factor is approximately 1:1. In one embodiment of the disclosed method the dilution factor is approximately 1:2. In one embodiment of the disclosed method the dilution factor is approximately 1:3. In one embodiment of the disclosed method the dilution factor is approximately 1:4. In one embodiment of the disclosed method the dilution factor is approximately 1:5. In one embodiment of the disclosed method the dilution factor is approximately 1:6. In one embodiment of the disclosed method the dilution factor is approximately 1:7. In one embodiment of the disclosed method the dilution factor is approximately 1:8. In one embodiment of the disclosed method the dilution factor is approximately 1:9. In one embodiment of the disclosed method the dilution factor is approximately 1:10. In one embodiment of the disclosed method the dilution factor is approximately 1:11. In one embodiment of the disclosed method the dilution factor is approximately 1:12. In one embodiment of the disclosed method the dilution factor is approximately 1:13. In one embodiment of the disclosed method the dilution factor is approximately 1:14. In one embodiment of the disclosed method the dilution factor is approximately 1:15. In one embodiment of the disclosed method the dilution factor is approximately 1:16. In one embodiment of the disclosed method the dilution factor is approximately 1:17. In one embodiment of the disclosed method the dilution factor is approximately 1:18. In one embodiment of the disclosed method the dilution factor is approximately 1:19. In one embodiment of the disclosed method the dilution factor is approximately 1:20. In one embodiment of the disclosed method the dilution factor is approximately 1:25. In one embodiment of the disclosed method the dilution factor is approximately 1:30. In one embodiment of the disclosed method the dilution factor is approximately 1:35. In one embodiment of the disclosed method the dilution factor is approximately 1:40. In one embodiment of the disclosed method the dilution factor is approximately 1:45. In one embodiment of the disclosed method the dilution factor is approximately 1:50. In one embodiment of the disclosed method the dilution factor is approximately 1:55. In one embodiment of the disclosed method the dilution factor is approximately 1:60. In one embodiment of the disclosed method the dilution factor is approximately 1:65. In one embodiment of the disclosed method the dilution factor is approximately 1:70. In one embodiment of the disclosed method the dilution factor is approximately 1:75. In one embodiment of the disclosed method the dilution factor is approximately 1:80. In one embodiment of the disclosed method the dilution factor is approximately 1:85. In one embodiment of the disclosed method the dilution factor is approximately 1:90. In one embodiment of the disclosed method the dilution factor is approximately 1:95. In one embodiment of the disclosed method the dilution factor is approximately 1:100.

Sample Preparation

In one embodiment of the disclosed method the sample is unfrozen. In one embodiment of the disclosed method the sample is frozen and then the DNA is extracted from the sample. In one embodiment of the disclosed method the sample is unfrozen and the DNA is extracted from the sample.

Sample Material

In one embodiment of the disclosed method the sample is a body fluid. In one embodiment of the disclosed method the sample is blood. In one embodiment of the disclosed method the sample is plasma. In one embodiment of the disclosed method the sample is urine. In one embodiment of the disclosed method the sample is sweat. In one embodiment of the disclosed method the sample is tears. In one embodiment of the disclosed method the sample is salvia. In one embodiment of the disclosed method the sample is seminal fluid. In one embodiment of the disclosed method the sample is vaginal fluid. In one embodiment of the disclosed method the sample is fecal cells. In one embodiment of the disclosed method the sample is feces.

In one embodiment of the disclosed method the sample is a tissue. In one embodiment of the disclosed method the sample is a cell. In one embodiment of the disclosed method the sample is a combination of tissue and cells. In one embodiment of the disclosed method the sample is cells. In one embodiment of the disclosed method the sample is red blood cells. In one embodiment of the disclosed method the sample is white blood cells. In one embodiment of the disclosed method the sample is lymphocytes. In one embodiment of the disclosed method the sample is platelets. In one embodiment of the disclosed method the sample is cells that centrifuge with red blood cells. In one embodiment of the disclosed method the sample is skin. In one embodiment of the disclosed method the sample is buccal cells. In one embodiment of the disclosed method the sample is nasal cells. In one embodiment of the disclosed method the sample is hair follicles. In one embodiment of the disclosed method the sample is ectoderm cells. In one embodiment of the disclosed method the sample is endoderm cells. In one embodiment of the disclosed method the sample is mesoderm cells. In one embodiment of the disclosed method the sample is sperm. In one embodiment of the disclosed method the sample is oocytes. In one embodiment of the disclosed method the sample is ovum. In one embodiment of the disclosed method the sample is eggs. In one embodiment of the disclosed method the sample is gametocytes. In one embodiment of the disclosed method the sample is stem cells. In one embodiment of the disclosed method the sample is cloned cells. In one embodiment of the disclosed method the sample is derived cells.

In one embodiment of the disclosed method the sample is body part. In one embodiment of the disclosed method the sample is a hand. In one embodiment of the disclosed method the sample is a finger. In one embodiment of the disclosed method the sample is an eye. In one embodiment of the disclosed method the sample is a hair. In one embodiment of the disclosed method the sample is a foot. In one embodiment of the disclosed method the sample is toe. In one embodiment of the disclosed method the sample is a face. In one embodiment of the disclosed method the sample is a palm. In one embodiment of the disclosed method the sample is a mouth. In one embodiment of the disclosed method the sample is a cheek. In one embodiment of the disclosed method the sample is a lip. In one embodiment of the disclosed method the sample is an arm. In one embodiment of the disclosed method the sample is a leg.

Sample Solution Content

In one embodiment of the disclosed method the sample is filtered. In one embodiment of the disclosed method the sample is a solution containing DNA and the solution is filtered. In one embodiment of the disclosed method the sample is a solution containing RNA and the solution is filtered.

In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 100 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 95 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 90 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 85 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 80 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 75 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 70 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 65 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 60 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 55 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 50 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 45 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 40 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 35 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 30 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 25 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 20 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 15 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 10 nm porosity. In one embodiment of the disclosed method the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 5 nm porosity.

PCR Primers

In one embodiment of the disclosed method the sample is analyzed with PCR primers. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Gag. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Pol. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Env. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Tat. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Rev. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Nef. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Vif. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Vpr. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Vpu. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV sequence and the HIV sequence is LTR. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV sequence and the HIV sequence is double LTR. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV gene of a HIV variant. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of at least one HIV gene. In one embodiment of the disclosed method the sample is analyzed with PCR primers of a combination of HIV genes. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a partial nucleotide sequence of the HIV sequence. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a nucleotide sequence of the DNA translation of a HIV RNA.

Viruses

In one embodiment of the disclosed method the detected virus is the HIV virus. In one embodiment of the disclosed method the detected virus is the Chickenpox (Varicella) virus. In one embodiment of the disclosed method the detected virus is the Common cold virus. In one embodiment of the disclosed method the detected virus is the Cytomegalovirus. In one embodiment of the disclosed method the detected virus is the Colorado tick fever virus. In one embodiment of the disclosed method the detected virus is the Dengue fever virus. In one embodiment of the disclosed method the detected virus is the Ebola hemorrhagic fever virus. In one embodiment of the disclosed method the detected virus is the Hand, foot and mouth disease virus. In one embodiment of the disclosed method the detected virus is the Hepatitis virus. In one embodiment of the disclosed method the detected virus is the Herpes simplex virus. In one embodiment of the disclosed method the detected virus is the Herpes zoster virus. In one embodiment of the disclosed method the detected virus is the HPV virus. In one embodiment of the disclosed method the detected virus is the Influenza (Flu) virus. In one embodiment of the disclosed method the detected virus is the Lassa fever virus. In one embodiment of the disclosed method the detected virus is the Measles virus. In one embodiment of the disclosed method the detected virus is the Marburg hemorrhagic fever virus. In one embodiment of the disclosed method the detected virus is the Infectious mononucleosis virus. In one embodiment of the disclosed method the detected virus is the Mumps virus. In one embodiment of the disclosed method the detected virus is the Norovirus. In one embodiment of the disclosed method the detected virus is the Poliomyelitis virus. In one embodiment of the disclosed method the detected virus is the Progressive multifocal leukencephalopathy virus. In one embodiment of the disclosed method the detected virus is the Rabies virus. In one embodiment of the disclosed method the detected virus is the Rubella virus. In one embodiment of the disclosed method the detected virus is the SARS virus. In one embodiment of the disclosed method the detected virus is the Smallpox (Variola) virus. In one embodiment of the disclosed method the detected virus is the Viral encephalitis virus. In one embodiment of the disclosed method the detected virus is the Viral gastroenteritis virus. In one embodiment of the disclosed method the detected virus is the Viral meningitis virus. In one embodiment of the disclosed method the detected virus is the Viral pneumonia virus. In one embodiment of the disclosed method the detected virus is the West Nile disease virus. In one embodiment of the disclosed method the detected virus is the Yellow fever virus.

Pathogenic Particle

In one embodiment of the disclosed method the pathogenic particle is a fungal cell. In one embodiment of the disclosed method the pathogenic particle is a bacteria. In one embodiment of the disclosed method the pathogenic particle is a virus.

Pathogenic Infection

In one embodiment of the disclosed method the pathogenic infection is a fungal infection. In one embodiment of the disclosed method the pathogenic infection is a bacterial infection. In one embodiment of the disclosed method the pathogenic infection is a viral infection.

Embodiments of the Disclosed Composition Time Period of Vortexing

In one embodiment of the disclosed composition the time period of vortexing is at least 1 second. In one embodiment of the disclosed composition the time period of vortexing is at least 2 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 3 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 4 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 5 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 6 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 7 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 8 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 9 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 10 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 11 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 12 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 13 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 14 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 15 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 16 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 17 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 18 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 19 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 20 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 25 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 30 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 35 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 40 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 45 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 50 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 55 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 60 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 90 seconds. In one embodiment of the disclosed composition the time period of vortexing is zit least 120 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 150 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 180 seconds. In one embodiment of the disclosed composition the time period of vortexing is at least 5 minutes. In one embodiment of the disclosed composition the time period of vortexing is at least 10 minutes.

In one embodiment of the disclosed composition the time period of vortexing is more than 1 second. In one embodiment of the disclosed composition the time period of vortexing is more than 2 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 3 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 4 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 5 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 6 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 7 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 8 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 9 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 10 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 11 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 12 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 13 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 14 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 15 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 16 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 17 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 18 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 19 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 20 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 25 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 30 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 35 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 40 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 45 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 50 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 55 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 60 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 90 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 120 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 150 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 180 seconds. In one embodiment of the disclosed composition the time period of vortexing is more than 5 minutes. In one embodiment of the disclosed composition the lime period of vortexing is more than 10 minutes.

In one embodiment of the disclosed composition the time period of vortexing is approximately 1 second. In one embodiment of the disclosed composition the time period of vortexing is approximately 2 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 3 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 4 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 5 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 6 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 7 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 8 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 9 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 10 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 11 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 12 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 13 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 14 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 15 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 16 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 17 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 18 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 19 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 20 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 25 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 30 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 35 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 40 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 45 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 50 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 55 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 60 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 90 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 120 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 150 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 180 seconds. In one embodiment of the disclosed composition the time period of vortexing is approximately 5 minutes. In one embodiment of the disclosed composition the time period of vortexing is approximately 10 minutes.

In one embodiment of the disclosed composition the time period of vortexing is 1 second. In one embodiment of the disclosed composition the time period of vortexing is 2 seconds. In one embodiment of the disclosed composition the time period of vortexing is 3 seconds. In one embodiment of the disclosed composition the time period of vortexing is 4 seconds. In one embodiment of the disclosed composition the time period of vortexing is 5 seconds. In one embodiment of the disclosed composition the time period of vortexing is 6 seconds. In one embodiment of the disclosed composition the time period of vortexing is 7 seconds. In one embodiment of the disclosed composition the time period of vortexing is 8 seconds. In one embodiment of the disclosed composition the time period of vortexing is 9 seconds. In one embodiment of the disclosed composition the time period of vortexing is 10 seconds. In one embodiment of the disclosed composition the time period of vortexing is 11 seconds. In one embodiment of the disclosed composition the time period of vortexing is 12 seconds. In one embodiment of the disclosed composition the time period of vortexing is 13 seconds. In one embodiment of the disclosed composition the time period of vortexing is 14 seconds. In one embodiment of the disclosed composition the time period of vortexing is 15 seconds. In one embodiment of the disclosed composition the time period of vortexing is 16 seconds. In one embodiment of the disclosed composition the time period of vortexing is 17 seconds. In one embodiment of the disclosed composition the time period of vortexing is 18 seconds. In one embodiment of the disclosed composition the time period of vortexing is 19 seconds. In one embodiment of the disclosed composition the time period of vortexing is 20 seconds. In one embodiment of the disclosed composition the time period of vortexing is 25 seconds. In one embodiment of the disclosed composition the time period of vortexing is 30 seconds. In one embodiment of the disclosed composition the time period of vortexing is 35 seconds. In one embodiment of the disclosed composition the time period of vortexing is 40 seconds. In one embodiment of the disclosed composition the time period of vortexing is 45 seconds. In one embodiment of the disclosed composition the time period of vortexing is 50 seconds. In one embodiment of the disclosed composition the time period of vortexing is 55 seconds. In one embodiment of the disclosed composition the time period of vortexing is 60 seconds. In one embodiment of the disclosed composition the time period of vortexing is 90 seconds. In one embodiment of the disclosed composition the time period of vortexing is 120 seconds. In one embodiment of the disclosed composition the time period of vortexing is 150 seconds. In one embodiment of the disclosed composition the time period of vortexing is 180 seconds. In one embodiment of the disclosed composition the time period of vortexing is 5 minutes. In one embodiment of the disclosed composition the time period of vortexing is 10 minutes.

Filtration, Dilution and Vortex Steps

In one embodiment of the disclosed composition the sample is diluted. In one embodiment of the disclosed composition the sample is serially diluted. In one embodiment of the disclosed composition the sample is diluted in series with the same dilution factor. In one embodiment of the disclosed composition the sample is diluted in series with different dilution factors. In one embodiment of the disclosed composition the sample is diluted and then vortexed. In one embodiment of the disclosed composition the sample is vortexed and then diluted.

In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated twice. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated three (3) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated four (4) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated five (5) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated six (6) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated seven (7) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated eight (8) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated nine (9) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated ten (10) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least twice. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least three (3) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least four (4) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least five (5) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least six (6) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least seven (7) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least eight (8) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least nine (9) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least ten (10) times.

In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated at least twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than twice. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than three (3) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than four (4) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than five (5) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than six (6) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than seven (7) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than eight (8) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than nine (9) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than ten (10) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and these steps are repeated more than twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated twice. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated three (3) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated four (4) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated five (5) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated six (6) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated seven (7) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated eight (8) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated nine (9) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated ten (10) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least twice. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least three (3) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least four (4) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least five (5) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least six (6) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least seven (7) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least eight (8) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least nine (9) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least ten (10) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than twice. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than three (3) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than four (4) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than five (5) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than six (6) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than seven (7) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than eight (8) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than nine (9) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than ten (10) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than twenty (20) times.

Filtration, Vortex and Dilution Steps

In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated twice. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated three (3) times. In one embodiment of the disclosed composition the sample is filtered, vortexed aid diluted, and these steps are repeated four (4) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated five (5) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated six (6) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated seven (7) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated eight (8) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated nine ‘,9) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated ten (10) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least twice. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least three (3) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least four (4) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least five (5) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least six (6) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least seven (7) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least eight (8) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least nine (9) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least ten (10) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated at least twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than twice. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than three (3) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than four (4) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than five (5) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than six (6) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than seven (7) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than eight (8) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than nine (9) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than ten (10) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and these steps are repeated more than twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated twice. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated three (3) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated four (4) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated five (5) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated six (6) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated seven (7) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated eight (8) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated nine (9) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated ten (10) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least twice. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least three (3) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least four (4) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least five (5) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least six (6) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and. diluted, and the “vortexed and diluted” steps are repeated at least seven (7) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least eight (8) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least nine (9) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least ten (10) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least twenty (20) times.

In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than twice. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than three (3) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than four (4) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than five (5) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than six (6) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than seven (7) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than eight (8) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than nine (9) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than ten (10) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than eleven (11) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than twelve (12) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than thirteen (13) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than fourteen (14) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than fifteen (15) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than sixteen (16) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than seventeen (17) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than eighteen (18) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than nineteen (19) times. In one embodiment of the disclosed composition the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than twenty (20) times.

Dilution Step In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻². In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻³. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻⁴. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻⁵. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻⁶. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻⁷. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻⁸. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻⁹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹⁰. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹¹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹². In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹³. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹⁴. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹⁵. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹⁶. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹⁷. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹⁸. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻¹⁹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted 10⁻²⁰.

In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻². In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻³. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻⁴. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻⁵. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻⁶. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻⁷. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻⁸. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻⁹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹⁰. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹¹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹². In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹³. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹⁴. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹⁵. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹⁶. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹⁷. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹⁸. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻¹⁹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted at least 10⁻²⁰.

In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻². In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻³. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻⁴. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻⁵. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻⁶. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻⁷. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻⁸. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻⁹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹⁰. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹¹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹². In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹³. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹⁴. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹⁵. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹⁶. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹⁷. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹⁸. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻¹⁹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted more than 10⁻²⁰.

In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻². In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻³. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻⁴. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻⁵. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻⁶. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻⁷. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻⁸. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻⁹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹⁰. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹¹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹². In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹³. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹⁴. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹⁵. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹⁶. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹⁷. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹⁸. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻¹⁹. In one embodiment of the disclosed composition the EMS is measured in a sample diluted approximately 10⁻²⁰.

Dilution Factor

In one embodiment of the disclosed composition the dilution factor is 1:1. In one embodiment of the disclosed composition the dilution factor is 1:2. In one embodiment of the disclosed composition the dilution factor is 1:3. In one embodiment of the disclosed composition the dilution factor is 1:4. In one embodiment of the disclosed composition the dilution factor is 1:5. In one embodiment of the disclosed composition the dilution factor is 1:6. In one embodiment of the disclosed composition the dilution factor is 1:7. In one embodiment of the disclosed composition the dilution factor is 1:8. In one embodiment of the disclosed composition the dilution factor is 1:9. In one embodiment of the disclosed composition the dilution factor is 1:10. In one embodiment of the disclosed composition the dilution factor is 1:11. In one embodiment of the disclosed composition the dilution factor is 1:12. In one embodiment of the disclosed composition the dilution factor is 1:13. In one embodiment of the disclosed composition the dilution factor is 1:14. In one embodiment of the disclosed composition the dilution factor is 1:15. In one embodiment of the disclosed composition the dilution factor is 1:16. In one embodiment of the disclosed composition the dilution factor is 1:17. In one embodiment of the disclosed composition the dilution factor is 1:18. In one embodiment of the disclosed composition the dilution factor is 1:19. In one embodiment of the disclosed composition the dilution factor is 1:20. In one embodiment of the disclosed composition the dilution factor is 1:25. In one embodiment of the disclosed composition the dilution factor is 1:30. In one embodiment of the disclosed composition the dilution factor is 1:35. In one embodiment of the disclosed composition the dilution factor is 1:40. In one embodiment of the disclosed composition the dilution factor is 1:45. In one embodiment of the disclosed composition the dilution factor is 1:50. In one embodiment of the disclosed composition the dilution factor is 1:55. In one embodiment of the disclosed composition the dilution factor is 1:60. In one embodiment of the disclosed composition the dilution factor is 1:65. In one embodiment of the disclosed composition the dilution factor is 1:70. In one embodiment of the disclosed composition the dilution factor is 1:75. In one embodiment of the disclosed composition the dilution factor is 1:80. In one embodiment of the disclosed composition the dilution factor is 1:85. In one embodiment of the disclosed composition the dilution factor is 1:90. In one embodiment of the disclosed composition the dilution factor is 1:95. In one embodiment of the disclosed composition the dilution factor is 1:100.

In one embodiment of the disclosed composition the dilution factor is at least 1:1. In one embodiment of the disclosed composition the dilution factor is at least 1:2. In one embodiment of the disclosed composition the dilution factor is at least 1:3. In one embodiment of the disclosed composition the dilution factor is at least 1:4. In one embodiment of the disclosed composition the dilution factor is at least 1:5. In one embodiment of the disclosed composition the dilution factor is at least 1:6. In one embodiment of the disclosed composition the dilution factor is at least 1:7. In one embodiment of the disclosed composition the dilution factor is at least 1:8. In one embodiment of the disclosed composition the dilution factor is at least 1:9. In one embodiment of the disclosed composition the dilution factor is at least 1:10. In one embodiment of the disclosed composition the dilution factor is at least 1:11. In one embodiment of the disclosed composition the dilution factor is at least 1:12. In one embodiment of the disclosed composition the dilution factor is at least 1:13. In one embodiment of the disclosed composition the dilution factor is at least 1:14. In one embodiment of the disclosed composition the dilution factor is at least 1:15. In one embodiment of the disclosed composition the dilution factor is at least 1:16. In one embodiment of the disclosed composition the dilution factor is at least 1:17. In one embodiment of the disclosed composition the dilution factor is at least 1:18. In one embodiment of the disclosed composition the dilution factor is at least 1:19. In one embodiment of the disclosed composition the dilution factor is at least 1:20. In one embodiment of the disclosed composition the dilution factor is at least 1:25. In one embodiment of the disclosed composition the dilution factor is at least 1:30. In one embodiment of the disclosed composition the dilution factor is at least 1:35. In one embodiment of the disclosed composition the dilution factor is at least 1:40. In one embodiment of the disclosed composition the dilution factor is at least 1:45. In one embodiment of the disclosed composition the dilution factor is at least 1:50. In one embodiment of the disclosed composition the dilution factor is at least 1:55. In one embodiment of the disclosed composition the dilution factor is at least 1:60. In one embodiment of the disclosed composition the dilution factor is at least 1:65. In one embodiment of the disclosed composition the dilution factor is at least 1:70. In one embodiment of the disclosed composition the dilution factor is at least 1:75. In one embodiment of the disclosed composition the dilution factor is at least 1:80. In one embodiment of the disclosed composition the dilution factor is at least 1:85. In one embodiment of the disclosed composition the dilution factor is at least 1:90. In one embodiment of the disclosed composition the dilution factor is at least 1:95. In one embodiment of the disclosed composition the dilution factor is at least 1:100.

In one embodiment of the disclosed composition the dilution factor is more than 1:1. In one embodiment of the disclosed composition the dilution factor is more than 1:2. In one embodiment of the disclosed composition the dilution factor is more than 1:3. In one embodiment of the disclosed composition the dilution factor is more than 1:4. In one embodiment of the disclosed composition the dilution factor is more than 1:5. In one embodiment of the disclosed composition the dilution factor is more than 1:6. In one embodiment of the disclosed composition the dilution factor is more than 1:7. In one embodiment of the disclosed composition the dilution factor is more than 1:8. In one embodiment of the disclosed composition the dilution factor is more than 1:9. In one embodiment of the disclosed composition the dilution factor is more than 1:10. In one embodiment of the disclosed composition the dilution factor is more than 1:11. In one embodiment of the disclosed composition the dilution factor is more than 1:12. In one embodiment of the disclosed composition the dilution factor is more than 1:13. In one embodiment of the disclosed composition the dilution factor is more than 1:14. In one embodiment of the disclosed composition the dilution factor is more than 1:15. In one embodiment of the disclosed composition the dilution factor is more than 1:16. In one embodiment of the disclosed composition the dilution factor is more than 1:17. In one embodiment of the disclosed composition the dilution factor is more than 1:18. In one embodiment of the disclosed composition the dilution factor is more than 1:19. In one embodiment of the disclosed composition the dilution factor is more than 1:20. In one embodiment of the disclosed composition the dilution factor is more than 1:25. In one embodiment of the disclosed composition the dilution factor is more than 1:30. In one embodiment of the disclosed composition the dilution factor is more than 1:35. In one embodiment of the disclosed composition the dilution factor is more than 1:40. In one embodiment of the disclosed composition the dilution factor is more than 1:45. In one embodiment of the disclosed composition the dilution factor is more than 1:50. In one embodiment of the disclosed composition the dilution factor is more than 1:55. In one embodiment of the disclosed composition the dilution factor is more than 1:60. In one embodiment of the disclosed composition the dilution factor is more than 1:65. In one embodiment of the disclosed composition the dilution factor is more than 1:70. In one embodiment of the disclosed composition the dilution factor is more than 1:75. In one embodiment of the disclosed composition the dilution factor is more than 1:80. In one embodiment of the disclosed composition the dilution factor is more than 1:85. In one embodiment of the disclosed composition the dilution factor is more than 1:90. In one embodiment of the disclosed composition the dilution factor is more than 1:95. In one embodiment of the disclosed composition the dilution factor is more than 1:100.

In one embodiment of the disclosed composition the dilution factor is approximately 1:1. In one embodiment of the disclosed composition the dilution factor is approximately 1:2. In one embodiment of the disclosed composition the dilution factor is approximately 1:3. In one embodiment of the disclosed composition the dilution factor is approximately 1:4. In one embodiment of the disclosed composition the dilution factor is approximately 1:5. In one embodiment of the disclosed composition the dilution factor is approximately 1:6. In one embodiment of the disclosed composition the dilution factor is approximately 1:7. In one embodiment of the disclosed composition the dilution factor is approximately 1:8. In one embodiment of the disclosed composition the dilution factor is approximately 1:9. In one embodiment of the disclosed composition the dilution factor is approximately 1:10. In one embodiment of the disclosed composition the dilution factor is approximately 1:11. In one embodiment of the disclosed composition the dilution factor is approximately 1:12. In one embodiment of the disclosed composition the dilution factor is approximately 1:13. In one embodiment of the disclosed composition the dilution factor is approximately 1:14. In one embodiment of the disclosed composition the dilution factor is approximately 1:15. In one embodiment of the disclosed composition the dilution factor is approximately 1:16. In one embodiment of the disclosed composition the dilution factor is approximately 1:17. In one embodiment of the disclosed composition the dilution factor is approximately 1:18. In one embodiment of the disclosed composition the dilution factor is approximately 1:19. In one embodiment of the disclosed composition the dilution factor is approximately 1:20. In one embodiment of the disclosed composition the dilution factor is approximately 1:25. In one embodiment of the disclosed composition the dilution factor is approximately 1:30. In one embodiment of the disclosed composition the dilution factor is approximately 1:35. In one embodiment of the disclosed composition the dilution factor is approximately 1:40. In one embodiment of the disclosed composition the dilution factor is approximately 1:45. In one embodiment of the disclosed composition the dilution factor is approximately 1:50. In one embodiment of the disclosed composition the dilution factor is approximately 1:55. In one embodiment of the disclosed composition the dilution factor is approximately 1:60. In one embodiment of the disclosed composition the dilution factor is approximately 1:65. In one embodiment of the disclosed composition the dilution factor is approximately 1:70. In one embodiment of the disclosed composition the dilution factor is approximately 1:75. In one embodiment of the disclosed composition the dilution factor is approximately 1:80. In one embodiment of the disclosed composition the dilution factor is approximately 1:85. In one embodiment of the disclosed composition the dilution factor is approximately 1:90. In one embodiment of the disclosed composition the dilution factor is approximately 1:95. In one embodiment of the disclosed composition the dilution factor is approximately 1:100.

Sample Preparation

In one embodiment of the disclosed composition the sample is unfrozen. In one embodiment of the disclosed composition the sample is frozen and then the DNA is extracted from the sample. In one embodiment of the disclosed composition the sample is unfrozen and the DNA is extracted from the sample.

Sample Material

In one embodiment of the disclosed composition the sample is a body fluid. In one embodiment of the disclosed composition the sample is blood. In one embodiment of the disclosed composition the sample is plasma. In one embodiment of the disclosed composition the sample is urine. In one embodiment of the disclosed composition the sample is sweat. In one embodiment of the disclosed composition the sample is tears. In one embodiment of the disclosed composition the sample is salvia. In one embodiment of the disclosed composition the sample is seminal fluid. In one embodiment of the disclosed composition the sample is vaginal fluid. In one embodiment of the disclosed composition the sample is fecal cells. In one embodiment of the disclosed composition the sample is feces.

In one embodiment of the disclosed composition the sample is a tissue. In one embodiment of the disclosed composition the sample is a cell. In one embodiment of the disclosed composition the sample is a combination of tissue and cells. In one embodiment of the disclosed composition the sample is cells. In one embodiment of the disclosed composition the sample is red blood cells. In one embodiment of the disclosed composition the sample is white blood cells. In one embodiment of the disclosed composition the sample is lymphocytes. In one embodiment of the disclosed composition the sample is platelets. In one embodiment of the disclosed composition the sample is cells that centrifuge with red blood cells. In one embodiment of the disclosed composition the sample is skin. In one embodiment of the disclosed composition the sample is buccal cells. In one embodiment of the disclosed composition the sample is nasal cells. In one embodiment of the disclosed composition the sample is hair follicles. In one embodiment of the disclosed composition the sample is ectoderm cells. In one embodiment of the disclosed composition the sample is endoderm cells. In one embodiment of the disclosed composition the sample is mesoderm cells. In one embodiment of the disclosed composition the sample is sperm. In one embodiment of the disclosed composition the sample is oocytes. In one embodiment of the disclosed composition the sample is ovum. In one embodiment of the disclosed composition the sample is eggs. In one embodiment of the disclosed composition the sample is gametocytes. In one embodiment of the disclosed composition the sample is stem cells. In one embodiment of the disclosed composition the sample is cloned cells. In one embodiment of the disclosed composition the sample is derived cells.

In one embodiment of the disclosed composition the sample is body part. In one embodiment of the disclosed composition the sample is a hand. In one embodiment of the disclosed composition the sample is a finger. In one embodiment of the disclosed composition the sample is an eye. In one embodiment of the disclosed composition the sample is a hair. In one embodiment of the disclosed composition the sample is a foot. In one embodiment of the disclosed composition the sample is toe. In one embodiment of the disclosed composition the sample is a face. In one embodiment of the disclosed composition the sample is a palm. In one embodiment of the disclosed composition the sample is a mouth. In one embodiment of the disclosed composition the sample is a cheek. In one embodiment of the disclosed composition the sample is a lip. In one embodiment of the disclosed composition the sample is an arm. In one embodiment of the disclosed composition the sample is a leg.

In one embodiment of the disclosed composition the sample is filtered. In one embodiment of the disclosed composition the sample is a solution containing DNA and the solution is filtered. In one embodiment of the disclosed composition the sample is a solution containing RNA and the solution is filtered.

Sample Solution Content

In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 100 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 95 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 90 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 85 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 80 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 75 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 70 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 65 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 60 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 55 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 50 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 45 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 40 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 35 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 30 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 25 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 20 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 15 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 10 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 5 nm porosity.

In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 100 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 95 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 90 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 85 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 80 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 75 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 70 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 65 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 60 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 55 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 50 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 45 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 40 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 35 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 30 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 25 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 20 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 15 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 10 nm porosity. In one embodiment of the disclosed composition the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 5 nm porosity.

Particle Size

In one embodiment of the disclosed composition the solution comprises particles less than 100 nm. In one embodiment of the disclosed composition the solution comprises particles less than 95 nm. In one embodiment of the disclosed composition the solution comprises particles less than 90 nm. In one embodiment of the disclosed composition the solution comprises particles less than 85 nm. In one embodiment of the disclosed composition the solution comprises particles less than 80 nm. In one embodiment of the disclosed composition the solution comprises particles less than 75 nm. In one embodiment of the disclosed composition the solution comprises particles less than 70 nm. In one embodiment of the disclosed composition the solution comprises particles less than 65 nm. In one embodiment of the disclosed composition the solution comprises particles less than 60 nm. In one embodiment of the disclosed composition the solution comprises particles less than 55 nm. In one embodiment of the disclosed composition the solution comprises particles less than 50 nm. In one embodiment of the disclosed composition the solution comprises particles less than 45 nm. In one embodiment of the disclosed composition the solution comprises particles less than 40 nm. In one embodiment of the disclosed composition the solution comprises particles less than 35 nm. In one embodiment of the disclosed composition the solution comprises particles less than 30 nm. In one embodiment of the disclosed composition the solution comprises particles less than 25 nm. In one embodiment of the disclosed composition the solution comprises particles less than 20 nm. In one embodiment of the disclosed composition the solution comprises particles less than 15 nm. In one embodiment of the disclosed composition the solution comprises particles less than 10 nm. In one embodiment of the disclosed composition the solution comprises particles less than 5 nm.

In one embodiment of the disclosed composition the solution comprises particles less than approximately 100 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 95 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 90 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 85 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 80 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 75 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 70 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 65 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 60 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 55 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 50 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 45 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 40 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 35 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 30 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 25 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 20 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 15 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 10 nm. In one embodiment of the disclosed composition the solution comprises particles less than approximately 5 nm.

In one embodiment of the disclosed composition the solution comprises particles not greater than 100 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 95 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 90 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 85 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 80 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 75 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 70 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 65 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 60 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 55 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 50 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 45 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 40 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 35 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 30 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 25 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 20 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 15 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 10 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than 5 nm.

In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 100 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 95 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 90 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 85 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 80 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 75 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 70 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 65 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 60 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 55 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 50 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 45 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 40 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 35 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 30 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 25 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 20 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 15 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 10 nm. In one embodiment of the disclosed composition the solution comprises particles not greater than approximately 5 nm.

Viruses

In one embodiment of the disclosed composition the detected virus is the HIV virus. In one embodiment of the disclosed composition the detected virus is the Chickenpox (Varicella) virus. In one embodiment of the disclosed composition the detected virus is the Common cold virus. In one embodiment of the disclosed composition the detected virus is the Cytomegalovirus. In one embodiment of the disclosed composition the detected virus is the Colorado tick fever virus. In one embodiment of the disclosed composition the detected virus is the Dengue fever virus. In one embodiment of the disclosed composition the detected virus is the Ebola hemorrhagic fever virus. In one embodiment of the disclosed composition the detected virus is the Hand, foot and mouth disease virus. In one embodiment of the disclosed composition the detected virus is the Hepatitis virus. In one embodiment of the disclosed composition the detected virus is the Herpes simplex virus. In one embodiment of the disclosed composition the detected virus is the Herpes zoster virus. In one embodiment of the disclosed composition the detected virus is the HPV virus. In one embodiment of the disclosed composition the detected virus is the Influenza (Flu) virus. In one embodiment of the disclosed composition the detected virus is the Lassa fever virus. In one embodiment of the disclosed composition the detected virus is the Measles virus. In one embodiment of the disclosed composition the detected virus is the Marburg hemorrhagic fever virus. In one embodiment of the disclosed composition the detected virus is the Infectious mononucleosis virus. In one embodiment of the disclosed composition the detected virus is the Mumps virus. In one embodiment of the disclosed composition the detected virus is the Norovirus. In one embodiment of the disclosed composition the detected virus is the Poliomyelitis virus. In one embodiment of the disclosed composition the detected virus is the Progressive multifocal leukencephalopathy virus. In one embodiment of the disclosed composition the detected virus is the Rabies virus. In one embodiment of the disclosed composition the detected virus is the Rubella virus. In one embodiment of the disclosed composition the detected virus is the SARS virus. In one embodiment of the disclosed composition the detected virus is the Smallpox (Variola) virus. In one embodiment of the disclosed composition the detected virus is the Viral encephalitis virus. In one embodiment of the disclosed composition the detected virus is the Viral gastroenteritis virus. In one embodiment of the disclosed composition the detected virus is the Viral meningitis virus. In one embodiment of the disclosed composition the detected virus is the Viral pneumonia virus. In one embodiment of the disclosed composition the detected virus is the West Nile disease virus. In one embodiment of the disclosed composition the detected virus is the Yellow fever virus.

Pathogenic Particle

In one embodiment of the disclosed composition the pathogenic particle is a fungal cell. In one embodiment of the disclosed composition the pathogenic particle is a bacterium.

In one embodiment of the disclosed composition the pathogenic particle is a virus.

Pathogenic Infection

In one embodiment of the disclosed composition the pathogenic infection is a fungal infection. In one embodiment of the disclosed composition the pathogenic infection is a bacterial infection. In one embodiment of the disclosed composition the pathogenic infection is a viral infection.

Embodiments of Disclosed Apparatus Time Period of Vortexing

In one embodiment of the disclosed apparatus the time period of vortexing is at least 1 second. In one embodiment of the disclosed apparatus the time period of vortexing is at least 2 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 3 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 4 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 5 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 6 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 7 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 8 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 9 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 10 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 11 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 12 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 13 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 14 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 15 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 16 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 17 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 18 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 19 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 20 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 25 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 30 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 35 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 40 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 45 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 50 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 55 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 60 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 90 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 120 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 150 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 180 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is at least 5 minutes. In one embodiment of the disclosed apparatus the time period of vortexing is at least 10 minutes.

In one embodiment of the disclosed apparatus the time period of vortexing is more than 1 second. In one embodiment of the disclosed apparatus the time period of vortexing is more than 2 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 3 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 4 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 5 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 6 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 7 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 8 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 9 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 10 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 11 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 12 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 13 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 14 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 15 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 16 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 17 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 18 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 19 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 20 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 25 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 30 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 35 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 40 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 45 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 50 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 55 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 60 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 90 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 120 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 150 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 180 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is more than 5 minutes. In one embodiment of the disclosed apparatus the time period of vortexing is more than 10 minutes.

In one embodiment of the disclosed apparatus the time period of vortexing is approximately 1 second. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 2 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 3 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 4 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 5 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 6 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 7 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 8 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 9 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 10 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 11 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 12 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 13 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 14 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 15 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 16 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 17 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 18 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 19 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 20 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 25 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 30 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 35 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 40 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 45 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 50 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 55 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 60 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 90 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 120 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 150 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 180 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 5 minutes. In one embodiment of the disclosed apparatus the time period of vortexing is approximately 10 minutes.

In one embodiment of the disclosed apparatus the time period of vortexing is 1 second. In one embodiment of the disclosed apparatus the time period of vortexing is 2 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 3 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 4 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 5 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 6 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 7 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 8 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 9 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 10 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 11 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 12 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 13 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 14 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 15 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 16 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 17 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 18 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 19 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 20 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 25 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 30 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 35 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 40 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 45 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 50 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 55 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 60 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 90 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 120 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 150 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 180 seconds. In one embodiment of the disclosed apparatus the time period of vortexing is 5 minutes. In one embodiment of the disclosed apparatus the time period of vortexing is 10 minutes.

Filtration, Dilution and Vortex Steps

In one embodiment of the disclosed apparatus the sample is diluted. In one embodiment of the disclosed apparatus the sample is serially diluted. In one embodiment of the disclosed apparatus the sample is diluted in series with the same dilution factor. In one embodiment of the disclosed apparatus the sample is diluted in series with different dilution factors. In one embodiment of the disclosed apparatus the sample is diluted and then vortexed. In one embodiment of the disclosed apparatus the sample is vortexed and then diluted.

In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated twice. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated eighteen (18) times. En one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least twice. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated at least twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than twice. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than sixteen (16) times. In one embodiment of the disclosed apparatus the sample is, filtered, diluted and vortexed, and these steps are repeated more than seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and these steps are repeated more than twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated twice. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least twice. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated at least twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, in that order. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than twice. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, diluted and vortexed, and the “diluted and vortexed” steps are repeated more than twenty (20) times.

Filtration, Vortex and Dilution Steps

In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated twice. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least twice. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least five (5) times In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated at least twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than twice. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and these steps are repeated more than twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated twice. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated three (3) times, In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least twice. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated at least twenty (20) times.

In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, in that order. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than twice. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than three (3) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than four (4) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than five (5) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than six (6) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than seven (7) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than eight (8) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than nine (9) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than ten (10) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than eleven (11) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than twelve (12) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than thirteen (13) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than fourteen (14) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than fifteen (15) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than sixteen (16) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than seventeen (17) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than eighteen (18) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than nineteen (19) times. In one embodiment of the disclosed apparatus the sample is filtered, vortexed and diluted, and the “vortexed and diluted” steps are repeated more than twenty (20) times.

Dilution Step

In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻². In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻³. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻⁴. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻⁵. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻⁶. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻⁷. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻⁸. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻⁹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹⁰. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹¹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹². In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹³. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹⁴. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹⁵. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹⁶. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹⁷. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹⁸. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻¹⁹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted 10⁻²⁰.

In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻². In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻³. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻⁴. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻⁵. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻⁶. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻⁷. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻⁸. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻⁹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹⁰. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹¹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹². In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹³. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹⁴. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹⁵. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹⁶. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹⁷. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹⁸. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻¹⁹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted at least 10⁻²⁰.

In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻². In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻³. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻⁴. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻⁵. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻⁶. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻⁷. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻⁸. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻⁹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹⁰. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹¹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹². In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹³. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹⁴. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10¹⁵. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹⁶. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹⁷. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹⁸. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻¹⁹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted more than 10⁻²⁰.

In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻². In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻³. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻⁴. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻⁵. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻⁶. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻⁷. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻⁸. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻⁹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹⁰. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹¹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹². In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹³. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹⁴. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹⁵. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹⁶. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹⁷. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹⁸. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻¹⁹. In one embodiment of the disclosed apparatus the EMS is measured in a sample diluted approximately 10⁻²⁰.

Dilution Factor

In one embodiment of the disclosed apparatus the dilution factor is 1:1. In one embodiment of the disclosed apparatus the dilution factor is 1:2. In one embodiment of the disclosed apparatus the dilution factor is 1:3. In one embodiment of the disclosed apparatus the dilution factor is 1:4. In one embodiment of the disclosed apparatus the dilution factor is 1:5. In one embodiment of the disclosed apparatus the dilution factor is 1:6. In one embodiment of the disclosed apparatus the dilution factor is 1:7. In one embodiment of the disclosed apparatus the dilution factor is 1:8. In one embodiment of the disclosed apparatus the dilution factor is 1:9. In one embodiment of the disclosed apparatus the dilution factor is 1:10. In one embodiment of the disclosed apparatus the dilution factor is 1:11. In one embodiment of the disclosed apparatus the dilution factor is 1:12. In one embodiment of the disclosed apparatus the dilution factor is 1:13. In one embodiment of the disclosed apparatus the dilution factor is 1:14. In one embodiment of the disclosed apparatus the dilution factor is 1:15. In one embodiment of the disclosed apparatus the dilution factor is 1:16. In one embodiment of the disclosed apparatus the dilution factor is 1:17. In one embodiment of the disclosed apparatus the dilution factor is 1:18. In one embodiment of the disclosed apparatus the dilution factor is 1:19. In one embodiment of the disclosed apparatus the dilution factor is 1:20. In one embodiment of the disclosed apparatus the dilution factor is 1:25. In one embodiment of the disclosed apparatus the dilution factor is 1:30. In one embodiment of the disclosed apparatus the dilution factor is 1:35. In one embodiment of the disclosed apparatus the dilution factor is 1:40. In one embodiment of the disclosed apparatus the dilution factor is 1:45. In one embodiment of the disclosed apparatus the dilution factor is 1:50. In one embodiment of the disclosed apparatus the dilution factor is 1:55. In one embodiment of the disclosed apparatus the dilution factor is 1:60. In one embodiment of the disclosed apparatus the dilution factor is 1:65. In one embodiment of the disclosed apparatus the dilution factor is 1:70. In one embodiment of the disclosed apparatus the dilution factor is 1:75. In one embodiment of the disclosed apparatus the dilution factor is 1:80. In one embodiment of the disclosed apparatus the dilution factor is 1:85. In one embodiment of the disclosed apparatus the dilution factor is 1:90. In one embodiment of the disclosed apparatus the dilution factor is 1:95. In one embodiment of the disclosed apparatus the dilution factor is 1:100.

In one embodiment of the disclosed apparatus the dilution factor is at least 1:1. In one embodiment of the disclosed apparatus the dilution factor is at least 1:2. In one embodiment of the disclosed apparatus the dilution factor is at least 1:3. In one embodiment of the disclosed apparatus the dilution factor is at least 1:4. In one embodiment of the disclosed apparatus the dilution factor is at least 1:5. In one embodiment of the disclosed apparatus the dilution factor is at least 1:6. In one embodiment of the disclosed apparatus the dilution factor is at least 1:7. In one embodiment of the disclosed apparatus the dilution factor is at least 1:8. In one embodiment of the disclosed apparatus the dilution factor is at least 1:9. In one embodiment of the disclosed apparatus the dilution factor is at least 1:10. In one embodiment of the disclosed apparatus the dilution factor is at least 1:11. In one embodiment of the disclosed apparatus the dilution factor is at least 1:12. In one embodiment of the disclosed apparatus the dilution factor is at least 1:13. In one embodiment of the disclosed apparatus the dilution factor is at least 1:14. In one embodiment of the disclosed apparatus the dilution factor is at least 1:15. In one embodiment of the disclosed apparatus the dilution factor is at least 1:16. In one embodiment of the disclosed apparatus the dilution factor is at least 1:17. In one embodiment of the disclosed apparatus the dilution factor is at least 1:18. In one embodiment of the disclosed apparatus the dilution factor is at least 1:19. In one embodiment of the disclosed apparatus the dilution factor is at least 1:20. In one embodiment of the disclosed apparatus the dilution factor is at least 1:25. In one embodiment of the disclosed apparatus the dilution factor is at least 1:30. In one embodiment of the disclosed apparatus the dilution factor is at least 1:35. In one embodiment of the disclosed apparatus the dilution factor is at least 1:40. In one embodiment of the disclosed apparatus the dilution factor is at least 1:45. In one embodiment of the disclosed apparatus the dilution factor is at least 1:50. In one embodiment of the disclosed apparatus the dilution factor is at least 1:55. In one embodiment of the disclosed apparatus the dilution factor is at least 1:60. In one embodiment of the disclosed apparatus the dilution factor is at least 1:65. In one embodiment of the disclosed apparatus the dilution factor is at least 1:70. In one embodiment of the disclosed apparatus the dilution factor is at least 1:75. In one embodiment of the disclosed apparatus the dilution factor is at least 1:80. In one embodiment of the disclosed apparatus the dilution factor is at least 1:85. In one embodiment of the disclosed apparatus the dilution factor is at least 1:90. In one embodiment of the disclosed apparatus the dilution factor is at least 1:95. In one embodiment of the disclosed apparatus the dilution factor is at least 1:100.

In one embodiment of the disclosed apparatus the dilution factor is more than 1:1. In one embodiment of the disclosed apparatus the dilution factor is more than 1:2. In one embodiment of the disclosed apparatus the dilution factor is more than 1:3. In one embodiment of the disclosed apparatus the dilution factor is more than 1:4. In one embodiment of the disclosed apparatus the dilution factor is more than 1:5. In one embodiment of the disclosed apparatus the dilution factor is more than 1:6. In one embodiment of the disclosed apparatus the dilution factor is more than 1:7. In one embodiment of the disclosed apparatus the dilution factor is more than 1:8. In one embodiment of the disclosed apparatus the dilution factor is more than 1:9. In one embodiment of the disclosed apparatus the dilution factor is more than 1:10. In one embodiment of the disclosed apparatus the dilution factor is more than 1:11. In one embodiment of the disclosed apparatus the dilution factor is more than 1:12. In one embodiment of the disclosed apparatus the dilution factor is more than 1:13. In one embodiment of the disclosed apparatus the dilution factor is more than 1:14. In one embodiment of the disclosed apparatus the dilution factor is more than 1:15. In one embodiment of the disclosed apparatus the dilution factor is more than 1:16. In one embodiment of the disclosed apparatus the dilution factor is more than 1:17. In one embodiment of the disclosed apparatus the dilution factor is more than 1:18. In one embodiment of the disclosed apparatus the dilution factor is more than 1:19. In one embodiment of the disclosed apparatus the dilution factor is more than 1:20. In one embodiment of the disclosed apparatus the dilution factor is more than 1:25. In one embodiment of the disclosed apparatus the dilution factor is more than 1:30. In one embodiment of the disclosed apparatus the dilution factor is more than 1:35. In one embodiment of the disclosed apparatus the dilution factor is more than 1:40. In one embodiment of the disclosed apparatus the dilution factor is more than 1:45. In one embodiment of the disclosed apparatus the dilution factor is more than 1:50. In one embodiment of the disclosed apparatus the dilution factor is more than 1:55. In one embodiment of the disclosed apparatus the dilution factor is more than 1:60. In one embodiment of the disclosed apparatus the dilution factor is more than 1:65. In one embodiment of the disclosed apparatus the dilution factor is more than 1:70. In one embodiment of the disclosed apparatus the dilution factor is more than 1:75. In one embodiment of the disclosed apparatus the dilution factor is more than 1:80. In one embodiment of the disclosed apparatus the dilution factor is more than 1:85. In one embodiment of the disclosed apparatus the dilution factor is more than 1:90. In one embodiment of the disclosed apparatus the dilution factor is more than 1:95. In one embodiment of the disclosed apparatus the dilution factor is more than 1:100.

In one embodiment of the disclosed apparatus the dilution factor is approximately 1:1. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:2. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:3. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:4. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:5. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:6. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:7. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:8. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:9. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:10. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:11. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:12. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:13. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:14. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:15. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:16. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:17. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:18. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:19. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:20. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:25. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:30. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:35. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:40. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:45. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:50. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:55. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:60. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:65. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:70. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:75. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:80. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:85. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:90. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:95. In one embodiment of the disclosed apparatus the dilution factor is approximately 1:100.

Sample Preparation

In one embodiment of the disclosed apparatus the sample is unfrozen. In one embodiment of the disclosed apparatus the sample is frozen and then the DNA is extracted from the sample. In one embodiment of the disclosed apparatus the sample is unfrozen and the DNA is extracted from the sample.

Sample Material

In one embodiment of the disclosed apparatus the sample is a body fluid. In one embodiment of the disclosed apparatus the sample is blood. In one embodiment of the disclosed apparatus the sample is plasma. In one embodiment of the disclosed apparatus the sample is urine. In one embodiment of the disclosed apparatus the sample is sweat. In one embodiment of the disclosed apparatus the sample is tears. In one embodiment of the disclosed apparatus the sample is salvia. In one embodiment of the disclosed apparatus the sample is seminal fluid. In one embodiment of the disclosed apparatus the sample is vaginal fluid. In one embodiment of the disclosed apparatus the sample is fecal cells. In one embodiment of the disclosed apparatus the sample is feces.

In one embodiment of the disclosed apparatus the sample is a tissue. In one embodiment of the disclosed apparatus the sample is a cell. In one embodiment of the disclosed apparatus the sample is a combination of tissue and cells. In one embodiment of the disclosed apparatus the sample is cells. In one embodiment of the disclosed apparatus the sample is red blood cells. In one embodiment of the disclosed apparatus the sample is white blood cells. In one embodiment of the disclosed apparatus the sample is lymphocytes. In one embodiment of the disclosed apparatus the sample is platelets. In one embodiment of the disclosed apparatus the sample is cells that centrifuge with red blood cells. In one embodiment of the disclosed apparatus the sample is skin. In one embodiment of the disclosed apparatus the sample is buccal cells. In one embodiment of the disclosed apparatus the sample is nasal cells. In one embodiment of the disclosed apparatus the sample is hair follicles. In one embodiment of the disclosed apparatus the sample is ectoderm cells. In one embodiment of the disclosed apparatus the sample is endoderm cells. In one embodiment of the disclosed apparatus the sample is mesoderm cells. In one embodiment of the disclosed apparatus the sample is sperm. In one embodiment of the disclosed apparatus the sample is oocytes. In one embodiment of the disclosed apparatus the sample is ovum. In one embodiment of the disclosed apparatus the sample is eggs. In one embodiment of the disclosed apparatus the sample is gametocytes. In one embodiment of the disclosed apparatus the sample is stem cells. In one embodiment of the disclosed apparatus the sample is cloned cells. In one embodiment of the disclosed apparatus the sample is derived cells.

In one embodiment of the disclosed apparatus the sample is body part. In one embodiment of the disclosed apparatus the sample is a hand. In one embodiment of the disclosed apparatus the sample is a finger. In one embodiment of the disclosed apparatus the sample is an eye. In one embodiment of the disclosed apparatus the sample is a hair. In one embodiment of the disclosed apparatus the sample is a foot. In one embodiment of the disclosed apparatus the sample is toe. In one embodiment of the disclosed apparatus the sample is a face. In one embodiment of the disclosed apparatus the sample is a palm. In one embodiment of the disclosed apparatus the sample is a mouth. In one embodiment of the disclosed apparatus the sample is a cheek. In one embodiment of the disclosed apparatus the sample is a lip. In one embodiment of the disclosed apparatus the sample is an arm. In one embodiment of the disclosed apparatus the sample is a leg.

Sample Solution Content

In one embodiment of the disclosed apparatus the sample is filtered. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and the solution is filtered. In one embodiment of the disclosed apparatus the sample is a solution containing RNA and the solution is filtered.

In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 100 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 95 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 90 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 85 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 80 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 75 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 70 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 65 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 60 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 55 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 50 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 45 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 40 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 35 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 30 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 25 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 20 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 15 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of at least 10 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter or at least 5 nm porosity.

In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 100 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 95 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 90 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 85 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 80 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 75 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 70 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 65 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 60 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 55 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 50 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 45 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 40 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 35 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 30 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 25 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 20 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 15 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 10 nm porosity. In one embodiment of the disclosed apparatus the sample is a solution containing DNA and/or RNA, and the solution is filtered with a filter of approximately 5 nm porosity.

Particle Size

In one embodiment of the disclosed apparatus the solution comprises particles less than 100 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 95 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 90 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 85 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 80 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 75 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 70 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 65 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 60 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 55 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 50 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 45 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 40 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 35 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 30 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 25 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 20 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 15 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 10 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than 5 nm.

In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 100 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 95 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 90 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 85 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 80 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 75 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 70 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 65 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 60 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 55 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 50 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 45 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 40 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 35 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 30 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 25 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 20 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 15 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 10 nm. In one embodiment of the disclosed apparatus the solution comprises particles less than approximately 5 nm.

In one embodiment of the disclosed apparatus the solution comprises particles not greater than 100 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 95 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 90 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 85 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 80 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 75 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 70 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 65 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 60 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 55 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 50 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 45 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 40 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 35 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 30 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 25 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 20 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 15 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 10 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than 5 nm.

In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 100 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 95 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 90 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 85 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 80 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 75 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 70 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 65 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 60 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 55 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 50 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 45 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 40 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 35 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 30 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 25 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 20 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 15 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 10 nm. In one embodiment of the disclosed apparatus the solution comprises particles not greater than approximately 5 nm.

PCR Primers

In one embodiment of the disclosed apparatus the sample is analyzed with PCR primers. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Gag. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Pol. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Env. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Tat. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Rev. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Nef. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Vif. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Vpr. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene and the HIV gene is Vpu. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV sequence and the HIV sequence is LTR. In one embodiment of the disclosed method the sample is analyzed with a PCR primer of a HIV sequence and the HIV sequence is double LTR. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a HIV gene of a HIV variant. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of at least one HIV gene. In one embodiment of the disclosed apparatus the sample is analyzed with PCR primers of a combination of HIV genes. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a partial nucleotide sequence of the HIV sequence. In one embodiment of the disclosed apparatus the sample is analyzed with a PCR primer of a nucleotide sequence of the DNA translation of a HIV RNA.

Viruses

In one embodiment of the disclosed apparatus the detected virus is the HIV virus. In one embodiment of the disclosed apparatus the detected virus is the Chickenpox (Varicella) virus. In one embodiment of the disclosed apparatus the detected virus is the Common cold virus. In one embodiment of the disclosed apparatus the detected virus is the Cytomegalovirus. In one embodiment of the disclosed apparatus the detected virus is the Colorado tick fever virus. In one embodiment of the disclosed apparatus the detected virus is the Dengue fever virus. In one embodiment of the disclosed apparatus the detected virus is the Ebola hemorrhagic fever virus. In one embodiment of the disclosed apparatus the detected virus is the Hand, foot and mouth disease virus. In one embodiment of the disclosed apparatus the detected virus is the Hepatitis virus. In one embodiment of the disclosed apparatus the detected virus is the Herpes simplex virus. In one embodiment of the disclosed apparatus the detected virus is the Herpes zoster virus. In one embodiment of the disclosed apparatus the detected virus is the HPV virus. In one embodiment of the disclosed apparatus the detected virus is the Influenza (Flu) virus. In one embodiment of the disclosed apparatus the detected virus is the Lassa fever virus. In one embodiment of the disclosed apparatus the detected virus is the Measles virus. In one embodiment of the disclosed apparatus the detected virus is the Marburg hemorrhagic fever virus. In one embodiment of the disclosed apparatus the detected virus is the Infectious mononucleosis virus. In one embodiment of the disclosed apparatus the detected virus is the Mumps virus. In one embodiment of the disclosed apparatus the detected virus is the Norovirus. In one embodiment of the disclosed apparatus the detected virus is the Poliomyelitis virus. In one embodiment of the disclosed apparatus the detected virus is the Progressive multifocal leukencephalopathy virus. In one embodiment of the disclosed apparatus the detected virus is the Rabies virus. In one embodiment of the disclosed apparatus the detected virus is the Rubella virus, In one embodiment of the disclosed apparatus the detected virus is the SARS virus. In one embodiment of the disclosed apparatus the detected virus is the Smallpox (Variola) virus. In one embodiment of the disclosed apparatus the detected virus is the Viral encephalitis virus, In one embodiment of the disclosed apparatus the detected virus is the Viral gastroenteritis virus. In one embodiment of the disclosed apparatus the detected virus is the Viral meningitis virus. In one embodiment of the disclosed apparatus the detected virus is the Viral pneumonia virus. In one embodiment of the disclosed apparatus the detected virus is the West Nile disease virus. In one embodiment of the disclosed apparatus the detected virus is the Yellow fever virus.

Pathogenic Particle

In one embodiment of the disclosed apparatus the pathogenic particle is a fungal cell. In one embodiment of the disclosed apparatus the pathogenic particle is a bacteria. In one embodiment of the disclosed apparatus the pathogenic particle is a virus.

Pathogenic Infection

In one embodiment of the disclosed apparatus the pathogenic infection is a fungal infection. In one embodiment of the disclosed apparatus the pathogenic infection is a bacterial infection. In one embodiment of the disclosed apparatus the pathogenic infection is a viral infection.

Modifications and Other Embodiments

Various modifications and variations of the described methods, procedures, techniques, and compositions as the concept of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed is not intended to be limited to such specific embodiments. Various modifications of the described modes for carrying out the invention which are obvious to those skilled in the medical, virological, immunological, pharmacological, molecular biological, physical sciences including electronic arts, or related fields are intended to be within the scope of the following claims.

INCORPORATION BY REFERENCE

Each document, patent application or patent publication cited by or referred to in this disclosure is incorporated by reference in its entirety. Any patent document to which this application claims priority is also incorporated by reference in its entirety. Specifically, priority document Provisional Application U.S. 61/186,610, filed Jun. 12, 2009, including all its attachments; Montagnier, et al., Intediscip. Sci. Comput. Life Sci., pp. 1-10 (2009); Montagnier, et al., Electromagnetic detection of HIV DNA in the blood of AIDS patients treated by antiretroviral therapy; and Montagnier, System and Method for the Analysis of DNA sequences in Biological Fluids are hereby incorporated by reference. 

1. A method for detecting a polynucleotide comprising: isolating nucleic acid from a sample; diluting the isolated nucleic acid in an aqueous solvent to produce a sample in a form suitable for measurement of low frequency electromagnetic emissions from nucleic acid or associated nanostructures in the sample over time; measuring or detecting said low frequency electromagnetic emissions over time; and determining the presence of said nucleic acid in the sample by detecting an electromagnetic emission signal (EMS) associated with said polynucleotide; wherein said signal is not produced by a sample isolated from an otherwise identical source that does not contain the nucleic acid.
 2. The method of claim 1, wherein said nucleic acid is DNA produced from an isolated viral polynucleotide or from a biological sample obtained from a subject infected with a virus encoding said viral polynucleotide using PCR or another nucleic acid amplification technique.
 3. The method of claim 1, wherein said nucleic acid is DNA produced from a viral polynucleotide isolated from a biological sample selected from the group consisting of blood, plasma, serum, seminal fluid, vaginal fluid, saliva, sweat, urine, and feces of said subject; or wherein said polynucleotide is obtained from a sample of portable water.
 4. The method of claim 1, wherein said nucleic acid is viral DNA encoded by human immunodeficiency virus.
 5. The method of claim 1, wherein said nucleic acid is viral DNA encoded by a human immunodeficiency virus isolated from a subject who is undergoing ART (anti-retroviral treatment), undergoing treatment with one or more inhibitors of reverse transcriptase, or is viral DNA encoded by a proviral form of a human immunodeficiency virus.
 6. The method of claim 1, wherein said nucleic acid is viral DNA encoded by infectious material isolated from a subject infected with human immunodeficiency virus that passes through a 20 nM filter.
 7. The method of claim 1, wherein said diluting step dilutes the nucleic acid by about 10⁻⁷ to 10⁻¹³ compared to its original concentration.
 8. The method of claim 1, wherein said measuring comprises placing the diluted nucleic acid near an antenna adapted to receive electromagnetic signals having a frequency approaching about 0 Hz to about 20 kHz and receiving the electromagnetic signals from the antenna.
 9. The method of claim 1, further comprising performing a time domain to frequency domain transformation on the measured or detected signal, optionally on the signal components of the measured or detected signal having frequencies between about 1 and 20,000 Hz.
 10. The method of claim 1, wherein measuring or detecting said low frequency electromagnetic signal emissions over time further comprises transmitting, outputting, displaying, printing, or producing a data structure representing the electromagnetic signal emissions or analyzed electromagnetic signal emissions; or further comprises outputting, displaying, or printing a three dimensional histogram of the electromagnetic signal emissions after Fourier transformation.
 11. The method of claim 1 for detecting an animal having a pathogenic infection, which comprises: a) obtaining a body fluid from an animal suspected of having a pathogenic infection, b) filtering the body fluid to obtain a filtered body fluid, c) serial diluting of the filtered body fluid until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid at a dilution of 1:9; d) measuring an EMS from the diluted body fluid in step d), e) analyzing the EMS, f) determining if the EMS corresponds to an EMS produced by a pathogen.
 12. The method of claim 1 for detecting a reservoir of human immunodeficiency virus in a subject comprising: (a) obtaining a sample of body fluid from an animal, filtering the sample, vortexing the sample, diluting the sample at a dilution of 1:9, measuring an EMS from the diluted sample, analyzing the EMS, and determining if the EMS corresponds to HIV virus; (b) obtaining a sample of body fluid from an animal, filtering the sample, serial diluting of the sample until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid at a dilution of 1:9; measuring an EMS from the diluted sample, analyzing the EMS, and determining if the EMS corresponds to HIV virus; (c) obtaining a sample of body fluid from an animal, filtering the sample, treating filtered sample with an RNase, vortexing the sample, diluting the sample at a dilution of 1:9, and analyzing diluted sample with RT-PCR; or (d) obtaining a sample of body fluid from an animal, filtering the sample, treating filtered sample with an RNase, serial diluting of the filtered sample with the RNase body until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid at a dilution of 1:9; analyzing diluted sample with RT-PCR; (e) obtaining a sample of body fluid from an animal, filtering the sample, treating filtered sample vortexing the sample, diluting the sample at a dilution of 1:9, and analyzing diluted sample using HIV primers with nested PCR; or (f) obtaining a sample of body fluid from an animal, filtering the sample, treating filtered sample, serial diluting of the filtered sample until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid at a dilution of 1:9; analyzing diluted sample using HIV primers with nested PCR.
 13. The method of claim 1 for determining the efficacy of a treatment for a pathogenic disease, to determine whether a subject has been cured of human immunodeficiency virus, detecting viral DNA in a subject with undetectable viral RNA, to assess eradication of a viral infection, or to confirm EMS generation by human immunodeficiency virus, respectively, comprising: (a) determining efficacy of treatment of a pathogenic infection comprising: measuring an EMS in a person corresponding to an EMS from a pathogenic particle; treating the person with a treatment for which an efficiency is being determined; measuring an EMS in the person treated with the treatment; and determining the relationship between the EMS before treatment and the EMS after treatment; (b) determining whether a subject has been cured of a human immunodeficiency virus infection comprising: measuring an EMS in a person corresponding to an EMS from a HIV virus; treating the person with a treatment for which a cure is expected; and not detecting an EMS in the person corresponding to the EMS from the HIV virus; (c) detecting viral DNA in a patient with undetectable viral RNA comprising: obtaining a sample of body fluid from a patient, filtering the sample, treating filtered sample with an RNase, serial diluting of the filtered sample with the RNase until obtaining a dilution to test for EMS; wherein, the serial diluting comprises multiple cycles of: vortexing the filtered body fluid and diluting the filtered body fluid at a dilution of 1:9; and analyzing diluted sample with RT-PCR; (d) assessing eradication of a viral infection based on reduction of viral DNA comprising: measuring an EMS in a person corresponding to an EMS from a viral DNA; treating the person with a treatment for which an efficiency is being determined; measuring an EMS in the person treated with the treatment; and determining the relationship between the EMS before treatment and the EMS after treatment; or (e) confirming EMS generation by human immunodeficiency virus comprising: obtaining a sample from a patient, filtering the sample, treating filtered sample with an RNase, vortexing the sample, diluting the sample at a dilution of 1:9, and amplifying the diluted sample with RT-PCR using a PCR primer for a HIV gene sequence
 14. A method for amplifying a DNA sample comprising: filtering a sample containing DNA, optionally, treating said sample with RNase, diluting or serially diluting the filtered sample, wherein said diluting or serially diluting comprises vigorous agitation or vortexing of the filtered sample, and amplifying DNA from said diluted sample by a method selected from the group consisting of polymerase chain reaction (PCR), nested-PCR, RT-PCR, nested RT-PCR, and other conventional DNA amplification methods.
 15. The method of claim 14, wherein said sample is treated with RNase after filtration.
 16. The method of claim 14, wherein said sample is serially diluted by a factor of 1/10 (one part sample to nine parts diluent) to a dilution of 10⁻⁷ to 10⁻¹³ based on the concentration of DNA in the original sample.
 17. The method of claim 14, wherein the sample is obtained from a subject infected with a human immunodeficiency virus.
 18. The method of claim 14, wherein said sample is material that passes through a 20 nM filter that is isolated from a subject infected with human immunodeficiency virus.
 19. A composition comprising a filtered, vortexed, diluted sample of DNA, prepared according to the method of claim 1, wherein the filtered, vortexed, diluted sample of DNA has a detectable electromagnetic signal.
 20. An apparatus to analyze a sample obtained from a subject having a pathogenic infection comprising: a sample loading device; a sample filtering device; a sample diluting device; a sample vortexing device; a sample measuring device for EMS; an EMS analyzer; and a data display device. 